PUBLICATION
Fli+ etsrp+ Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish
- Authors
- Chun, C.Z., Remadevi, I., Schupp, M.O., Samant, G.V., Pramanik, K., Wilkinson, G.A., and Ramchandran, R.
- ID
- ZDB-PUB-110317-17
- Date
- 2011
- Source
- PLoS One 6(2): e14732 (Journal)
- Registered Authors
- Chun, Chang Zoon, Ramchandran, Ramani, Samant, Ganesh, Schupp, Marcus
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Vessels/embryology*
- Blood Vessels/metabolism
- Cell Movement/physiology
- Cell Proliferation*
- Embryo, Nonmammalian
- Endothelium, Vascular/embryology
- Endothelium, Vascular/metabolism
- Endothelium, Vascular/physiology
- Gene Expression Regulation, Developmental
- Head/embryology
- Hematopoietic Stem Cells/metabolism
- Hematopoietic Stem Cells/physiology*
- Mesoderm/cytology*
- Mesoderm/metabolism
- Mesoderm/physiology
- Models, Biological
- Neovascularization, Physiologic/genetics
- Neovascularization, Physiologic/physiology
- Proto-Oncogene Protein c-fli-1/genetics
- Proto-Oncogene Protein c-fli-1/metabolism*
- Proto-Oncogene Protein c-fli-1/physiology
- Tail/embryology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology
- PubMed
- 21364913 Full text @ PLoS One
Citation
Chun, C.Z., Remadevi, I., Schupp, M.O., Samant, G.V., Pramanik, K., Wilkinson, G.A., and Ramchandran, R. (2011) Fli+ etsrp+ Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish. PLoS One. 6(2):e14732.
Abstract
BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood.
METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM.
CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.
METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM.
CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping