PUBLICATION
Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors
- Authors
- Alexander, M.S., Kawahara, G., Kho, A.T., Howell, M.H., Pusack, T.J., Myers, J.A., Montanaro, F., Zon, L.I., Guyon, J.R., and Kunkel, L.M.
- ID
- ZDB-PUB-110316-12
- Date
- 2011
- Source
- Muscle & nerve 43(5): 741-750 (Journal)
- Registered Authors
- Alexander, Matthew, Guyon, Jeff, Kawahara, Genri, Kunkel, Louis M., Zon, Leonard I.
- Keywords
- differentiation, muscle mutants, muscle stem cells, myogenic progenitors, myogenesis, transcriptome, zebrafish
- Datasets
- GEO:GSE19754
- MeSH Terms
-
- Aging/physiology*
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology
- Cells, Cultured
- Gene Expression Profiling/methods*
- Muscle Development/physiology
- Muscle, Skeletal/cytology
- Muscle, Skeletal/growth & development
- Muscle, Skeletal/physiology*
- Myoblasts/cytology
- Myoblasts/physiology*
- Stem Cells/cytology
- Stem Cells/physiology*
- Zebrafish
- PubMed
- 21337346 Full text @ Muscle Nerve
Citation
Alexander, M.S., Kawahara, G., Kho, A.T., Howell, M.H., Pusack, T.J., Myers, J.A., Montanaro, F., Zon, L.I., Guyon, J.R., and Kunkel, L.M. (2011) Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors. Muscle & nerve. 43(5):741-750.
Abstract
Introduction: Over the past 10 years, the use of zebrafish for scientific research in the area of muscle development has increased dramatically. Although several protocols exist for the isolation of adult myoblast progenitors from larger fish, no standardized protocol exists for the isolation of myogenic progenitors from adult zebrafish muscle. Methods: Using a variant of a mammalian myoblast isolation protocol, zebrafish muscle progenitors have been isolated from the total dorsal myotome. These zebrafish myoblast progenitors can be cultured for several passages and then differentiated into multinucleated, mature myotubes. Results: Transcriptome analysis of these cells during myogenic differentiation revealed a strong downregulation of pluripotency genes, while, conversely, showing an upregulation of myogenic signaling and structural genes. Conclusions: Together these studies provide a simple, yet detailed method for the isolation and culture of myogenic progenitors from adult zebrafish, while further promoting their therapeutic potential for the study of muscle disease and drug screening.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping