ZFIN ID: ZDB-PUB-110214-13
Zebrafish HSC70 promoter to express carp muscle-specific creatine kinase for acclimation under cold condition
Wu, C.L., Lin, T.H., Chang, T.L., Sun, H.W., Hui, C.F., and Wu, J.L.
Date: 2011
Source: Transgenic Research   20(6): 1217-26 (Journal)
Registered Authors: Sun, His-Wen, Wu, Jen-Leih
Keywords: Creatine kinase, Heat shock cognate, Hsc 70, Cold, Inducible promoter, Zebrafish
MeSH Terms:
  • Acclimatization*
  • Animals
  • Animals, Genetically Modified
  • Carps/genetics*
  • Cold Temperature
  • Creatine Kinase/metabolism*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/physiology
  • Enzyme Activation
  • Escherichia coli/genetics
  • Escherichia coli/metabolism
  • Gene Expression Regulation
  • HSC70 Heat-Shock Proteins/genetics*
  • HSC70 Heat-Shock Proteins/metabolism
  • Microinjections
  • Models, Animal
  • Plasmids/genetics
  • Plasmids/metabolism
  • Promoter Regions, Genetic*
  • Swimming/physiology
  • Transfection
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 21298478 Full text @ Transgenic. Res.
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ABSTRACT
Zebrafish (Danio rerio) is used as a model system for in vivo studies. To expand the research scope of physical, biochemical and physiological studies, a cold-tolerant model of zebrafish was developed. The common carp (Cyprinus carpio) muscle form of creatine kinase (CK, EC 2.7.3.2) can maintain enzymatic activity at a temperature of around 15°C. However, a cold-inducible promoter of zebrafish, hsc 70 (heat shock protein 70 cognate), is able to increase the expression of gene product by 9.8 fold at a temperature of 16°C. Therefore, the carp CK gene was promoted by hsc 70 and transfected into zebrafish embryos. Resulting transgenic zebrafish survived and could maintain its swimming behavior at 13°C, which was not possible with the wild-type zebrafish. The swimming distance of the transgenic fish was 42% greater than that of the wild type at 13°C. This new transgenic fish model is ideal for studies of ectothermal vertebrates in low-temperature environments.
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