PUBLICATION
Expression and function of the Ets transcription factor pea3 during formation of zebrafish pronephros
- Authors
- Chen, Q., Huang, S., Zhao, Q., Chen, R., and Zhang, A.
- ID
- ZDB-PUB-110103-16
- Date
- 2011
- Source
- Pediatric nephrology (Berlin, Germany) 26(3): 391-400 (Journal)
- Registered Authors
- Zhao, Qingshun
- Keywords
- pea3, Zebrafish, Pronephros, Nephrogenesis, erm, wt1a
- MeSH Terms
-
- Animals
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- In Situ Hybridization
- Kidney/embryology
- Kidney/metabolism*
- Oligonucleotides, Antisense/metabolism
- Organogenesis
- Polymerase Chain Reaction
- RNA, Messenger/metabolism
- Time Factors
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- WT1 Proteins/genetics
- WT1 Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21184241 Full text @ Pediatr. Nephrol.
Citation
Chen, Q., Huang, S., Zhao, Q., Chen, R., and Zhang, A. (2011) Expression and function of the Ets transcription factor pea3 during formation of zebrafish pronephros. Pediatric nephrology (Berlin, Germany). 26(3):391-400.
Abstract
Polyomavirus enhancer activator 3 (Pea3), belonging to the PEA3 subfamily of Ets transcription factors, is essential for certain organogenesis in mammals. Previously, we found that pea3 correlated with wt1 expression and may contribute to nephrogenesis in rats. Here, we observed that pea3 was mainly expressed in the zebrafish pronephric glomerulus. We further performed functional analyses by in situ hybridization of pea3 in zebrafish embryos after pea3 messenger RNA (mRNA) overexpression and inhibition of double-target genes (pea3 and erm, another member of the PEA3 subfamily) by antisense morpholino-oligonucleotides (MO). Overexpression of pea3 induced abnormal pronephrogenesis. However, MO-pea3 coinjected with MO-erm, but not alone, inhibited zebrafish pronephros development, and these defects were rescued by overexpression of the zebrafish wt1a gene. Thus, pea3 and erm are required for zebrafish pronephrogenesis and can functionally complement each other, and the wt1a gene may be one of their downstream targets.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping