PUBLICATION
Chapter 12 - Use of Flatbed Transparency Scanners in Zebrafish Research Versatile and Economical Adjuncts to Traditional Imaging Tools for the Danio rerio Laboratory
- Authors
- Lessman, C.A., Taylor, M.R., Orisme, W., and Carver, E.A.
- ID
- ZDB-PUB-101201-34
- Date
- 2010
- Source
- Methods in cell biology 100: 295-322 (Chapter)
- Registered Authors
- Lessman, Charles, Taylor, Michael R.
- Keywords
- Oocyte maturation, egg activation, embryo counts, motility assay, adult imaging, drug testing, toxicology, cancer
- MeSH Terms
-
- Animals
- Image Processing, Computer-Assisted/instrumentation*
- Image Processing, Computer-Assisted/methods
- Zebrafish/anatomy & histology*
- Zebrafish/embryology
- PubMed
- 21111223 Full text @ Meth. Cell. Biol.
Citation
Lessman, C.A., Taylor, M.R., Orisme, W., and Carver, E.A. (2010) Chapter 12 - Use of Flatbed Transparency Scanners in Zebrafish Research Versatile and Economical Adjuncts to Traditional Imaging Tools for the Danio rerio Laboratory. Methods in cell biology. 100:295-322.
Abstract
Flatbed transparency scanners are typically relegated to routine office tasks, yet they do offer a variety of potentially useful imaging tools for the zebrafish laboratory. These include motility screens, oocyte maturation and egg activation assays as well as counting and measuring tasks. When coupled with Macroscheduler (http://www.mjtnet.com) and ImageJ (http://rsbweb.nih.gov/ij), the scanner becomes a stable platform for imaging large arrays of zebrafish oocytes, embryos, larvae, and adults. Such large arrays are a prerequisite to the development of high-throughput screens for small molecules as potential therapeutic drugs in the treatment of many diseases including cancer and epilepsy. Thus the scanner may have a role in adapting zebrafish to future drug and mutagenesis screening. In this chapter, some of the uses of scanners are outlined to bring attention to the potentials of this simple-to-use, flexible, inexpensive device for the zebrafish research community.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping