PUBLICATION
SSDP cofactors regulate neural patterning and differentiation of specific axonal projections
- Authors
- Zhong, Z., Ma, H., Taniguchi-Ishigaki, N., Nagarajan, L., Becker, C.G., Bach, I., and Becker, T.
- ID
- ZDB-PUB-101115-7
- Date
- 2011
- Source
- Developmental Biology 349(2): 213-224 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas
- Keywords
- SSDP, CLIM, LIM homeodomain protein, Transcriptional cofactors, Neural patterning, Development of axonal projections, Protein interaction
- MeSH Terms
-
- Gene Expression Regulation, Developmental/physiology*
- Reverse Transcriptase Polymerase Chain Reaction
- Cell Differentiation/physiology
- Sensory Receptor Cells/metabolism
- Sensory Receptor Cells/physiology*
- Axons/physiology*
- DNA Primers/genetics
- Blotting, Western
- Zebrafish/embryology*
- Neurogenesis/physiology*
- Animals
- Immunohistochemistry
- Transcription Factors/metabolism*
- In Situ Hybridization
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- PubMed
- 21056553 Full text @ Dev. Biol.
Citation
Zhong, Z., Ma, H., Taniguchi-Ishigaki, N., Nagarajan, L., Becker, C.G., Bach, I., and Becker, T. (2011) SSDP cofactors regulate neural patterning and differentiation of specific axonal projections. Developmental Biology. 349(2):213-224.
Abstract
The developmental activity of LIM homeodomain transcription factors (LIM-HDs) is critically controlled by LIM domain-interacting cofactors of LIM-HDs (CLIM, NLI, LDB). CLIM cofactors associate with Single stranded DNA binding proteins (SSDPs, also known as SSBPs) thereby recruiting SSDP1 and/or SSDP2 to LIM-HD/CLIM complexes. Although evidence has been presented that SSDPs are important for the activity of specific LIM-HD/CLIM complexes, the developmental roles of SSDPs are unclear. We show that SSDP1a and SSDP1b mRNAs are widely expressed early during zebrafish development with conspicuous expression of SSDP1b in sensory trigeminal and Rohon-Beard neurons. SSDP1 and CLIM immunoreactivity co-localize in these neuronal cell types and in other structures. Over-expression of the N-terminal portion of SSDP1 (N-SSDP1), which contains the CLIM interaction domain, increases endogenous CLIM protein levels in vivo and impairs the formation of eyes and midbrain-hindbrain boundary. In addition, inhibition of SSDP1 via N-SSDP1 over-expression or SSDP1b knock down impairs trigeminal and Rohon-Beard sensory axon growth. We show that N-SSDP1 is able to partially rescue the inhibition of axon growth induced by a dominant-negative form of CLIM (DN-CLIM). These results reveal specific functions of SSDP in neural patterning and sensory axon growth, in part due to the stabilization of LIM-HD/CLIM complexes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping