PUBLICATION
Lnx-2b restricts gsc expression to the dorsal mesoderm by limiting Nodal and Bozozok activity
- Authors
- Ro, H., and Dawid, I.B.
- ID
- ZDB-PUB-101027-37
- Date
- 2010
- Source
- Biochemical and Biophysical Research Communications 402(4): 626-630 (Journal)
- Registered Authors
- Dawid, Igor B.
- Keywords
- Lnx-2b, Nodal, Bozozok, goosecoid, dorsal organizer, dorsal mesoderm
- MeSH Terms
-
- Animals
- Goosecoid Protein/biosynthesis*
- Ectoderm/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- Mesoderm/metabolism*
- Homeodomain Proteins/metabolism*
- Nodal Signaling Ligands/metabolism*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- PubMed
- 20971071 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ro, H., and Dawid, I.B. (2010) Lnx-2b restricts gsc expression to the dorsal mesoderm by limiting Nodal and Bozozok activity. Biochemical and Biophysical Research Communications. 402(4):626-630.
Abstract
Coordinated Nodal-related signals and Bozozok (Boz) activity are critical for the initial specification of dorsal mesoderm and anterior neuroectoderm during zebrafish embryogenesis. Overexpression of Boz expands gsc expression into the ventro-lateral marginal blastomeres where Nodal signaling is active, but is insufficient to induce ectopic gsc expression in the animal region. We found that overexpression of Boz together with depletion of Lnx-2b (previously named Lnx-like, Lnx-l), but not each manipulation alone, causes robust gsc expression in all blastomeres. Furthermore, Nodal-related signals are required for gsc expression in embryos with elevated Boz activity. Through targeted injection into single cells at the 128-cell stage we illustrate the role of maternally deposited Lnx-2b to restrict the expansion of gsc expression into the presumptive ectodermal region. This report provides a novel mechanism for limiting dorsal organizer specification to a defined region of the early zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping