ZFIN ID: ZDB-PUB-101027-37
Lnx-2b restricts gsc expression to the dorsal mesoderm by limiting Nodal and Bozozok activity
Ro, H., and Dawid, I.B.
Date: 2010
Source: Biochemical and Biophysical Research Communications   402(4): 626-630 (Journal)
Registered Authors: Dawid, Igor B.
Keywords: Lnx-2b, Nodal, Bozozok, goosecoid, dorsal organizer, dorsal mesoderm
MeSH Terms:
  • Animals
  • Ectoderm/metabolism
  • Goosecoid Protein/biosynthesis*
  • Homeodomain Proteins/metabolism*
  • Mesoderm/metabolism*
  • Nodal Signaling Ligands/metabolism*
  • Ubiquitin-Protein Ligases/genetics
  • Ubiquitin-Protein Ligases/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 20971071 Full text @ Biochem. Biophys. Res. Commun.
Coordinated Nodal-related signals and Bozozok (Boz) activity are critical for the initial specification of dorsal mesoderm and anterior neuroectoderm during zebrafish embryogenesis. Overexpression of Boz expands gsc expression into the ventro-lateral marginal blastomeres where Nodal signaling is active, but is insufficient to induce ectopic gsc expression in the animal region. We found that overexpression of Boz together with depletion of Lnx-2b (previously named Lnx-like, Lnx-l), but not each manipulation alone, causes robust gsc expression in all blastomeres. Furthermore, Nodal-related signals are required for gsc expression in embryos with elevated Boz activity. Through targeted injection into single cells at the 128-cell stage we illustrate the role of maternally deposited Lnx-2b to restrict the expansion of gsc expression into the presumptive ectodermal region. This report provides a novel mechanism for limiting dorsal organizer specification to a defined region of the early zebrafish embryo.