PUBLICATION

Thyrotroph embryonic factor regulates light-induced transcription of repair genes in zebrafish embryonic cells

Authors

Gavriouchkina, D., Fischer, S., Ivacevic, T., Stolte, J., Benes, V., and Dekens, M.P.

ID

ZDB-PUB-100929-9

Date

2010

Source

PLoS One 5(9): e12542 (Journal)

Registered Authors

Dekens, Marcus P.S.

Keywords

Embryos, Gene regulation, Zebrafish, Circadian rhythms, DNA transcription, DNA repair, Transcription factors, Stress signaling cascade

MeSH Terms

DNA Damage
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Light Signal Transduction/radiation effects
Gene Expression Regulation, Developmental/radiation effects
Transcription, Genetic*/radiation effects
Animals
Basic-Leucine Zipper Transcription Factors/genetics
Basic-Leucine Zipper Transcription Factors/metabolism*
Promoter Regions, Genetic/radiation effects
Response Elements/radiation effects
Ultraviolet Rays
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
DNA Repair/radiation effects*

(all 16)

PubMed

20830285 Full text @ PLoS One

Abstract

Numerous responses are triggered by light in the cell. How the light signal is detected and transduced into a cellular response is still an enigma. Each zebrafish cell has the capacity to directly detect light, making this organism particularly suitable for the study of light dependent transcription. To gain insight into the light signalling mechanism we identified genes that are activated by light exposure at an early embryonic stage, when specialised light sensing organs have not yet formed. We screened over 14,900 genes using micro-array GeneChips, and identified 19 light-induced genes that function primarily in light signalling, stress response, and DNA repair. Here we reveal that PAR Response Elements are present in all promoters of the light-induced genes, and demonstrate a pivotal role for the PAR bZip transcription factor Thyrotroph embryonic factor (Tef) in regulating the majority of light-induced genes. We show that tefbeta transcription is directly regulated by light while transcription of tefalpha is under circadian clock control at later stages of development. These data leads us to propose their involvement in light-induced UV tolerance in the zebrafish embryo.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cpdp	WT	constant light	90%-epiboly	whole organism	RTPCR	Fig. 1
cpdp	WT + MO2-tefa	constant light	90%-epiboly	whole organism	RTPCR	Fig. 5
cpdp	WT + MO2-tefa + MO3-tefa	constant light	90%-epiboly	whole organism	RTPCR	Fig. 5
cpdp	WT + MO3-tefa	constant light	90%-epiboly	whole organism	RTPCR	Fig. 5
cry1a	WT	constant light	Prim-5	optic cup	ISH	Fig. 2
cry1a	WT	constant light	Prim-5	whole organism anterior-posterior gradient	ISH	Fig. 2
cry1a	WT	constant light	90%-epiboly	whole organism	RTPCR	Fig. 1
cry1a	WT	constant dark	Prim-5	whole organism	ISH	Fig. 2
cry1a	WT + MO2-tefa	constant light	90%-epiboly	whole organism	RTPCR	Fig. 5
cry1a	WT + MO2-tefa + MO3-tefa	constant light	90%-epiboly	whole organism	RTPCR	Fig. 5

1 - 10 of 103

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	altered light dark cycle	90%-epiboly	positive regulation of DNA-templated transcription disrupted, abnormal	Fig. 5
WT + MO2-tefa	constant light	90%-epiboly	positive regulation of DNA-templated transcription disrupted, abnormal	Fig. 5
WT + MO3-tefa	constant light	90%-epiboly	positive regulation of DNA-templated transcription disrupted, abnormal	Fig. 5

1 - 3 of 3

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tefa	MO2-tefa	MRPHLNO
tefa	MO3-tefa	MRPHLNO

Fish

Fish
WT
WT + MO2-tefa
WT + MO2-tefa + MO3-tefa
WT + MO3-tefa

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Gavriouchkina et al., 2010 ZDB-PUB-100929-9 PMID:20830285

Thyrotroph embryonic factor regulates light-induced transcription of repair genes in zebrafish embryonic cells

Gavriouchkina et al., 2010

ZDB-PUB-100929-9
PMID:20830285