ZFIN ID: ZDB-PUB-100820-30
Imaging Cilia in Zebrafish
Jaffe, K.M., Thiberge, S.Y., Bisher, M.E., and Burdine, R.D.
Date: 2010
Source: Methods in cell biology   97C: 415-435 (Chapter)
Registered Authors: Burdine, Rebecca, Jaffe, Kimberly
Keywords: none
MeSH Terms:
  • Animals
  • Cilia/chemistry
  • Cilia/metabolism
  • Cilia/physiology
  • Cilia/ultrastructure*
  • Cryoultramicrotomy/methods
  • Embryo, Nonmammalian
  • Humans
  • Microscopy/methods*
  • Models, Biological
  • Movement/physiology
  • Zebrafish*/embryology
  • Zebrafish*/physiology
PubMed: 20719283 Full text @ Meth. Cell. Biol.
ABSTRACT
Research focused on cilia as extremely important cellular organelles has flourished in recent years. A thorough understanding of cilia regulation and function is critical, as disruptions of cilia structure and/or function have been linked to numerous human diseases and disorders. The tropical freshwater zebrafish is an excellent model organism in which to study cilia structure and function. We can readily image cilia and their motility in embryonic structures including Kupffer's vesicle during somite stages and the pronephros from 1 day postfertilization onward. Here, we describe how to image cilia by whole-mount immunofluorescence, transverse cryosection/immunohistochemistry, and transmission electron microscopy. We also describe how to obtain videos of cilia motility in living embryos.
ADDITIONAL INFORMATION No data available