PUBLICATION
Lack of Apobec2-related proteins causes a dystrophic muscle phenotype in zebrafish embryos
- Authors
- Etard, C., Roostalu, U., and Strähle, U.
- ID
- ZDB-PUB-100511-4
- Date
- 2010
- Source
- The Journal of cell biology 189(3): 527-539 (Journal)
- Registered Authors
- Etard, Christelle, Roostalu, Urmas, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Animals
- Cytidine Deaminase/genetics*
- Cytidine Deaminase/metabolism
- Embryo, Nonmammalian/metabolism*
- HSP90 Heat-Shock Proteins/genetics
- HSP90 Heat-Shock Proteins/metabolism
- Molecular Chaperones/genetics
- Molecular Chaperones/metabolism
- Muscle, Skeletal/embryology*
- Muscle, Skeletal/metabolism
- Phenotype*
- Type C Phospholipases/genetics
- Type C Phospholipases/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 20440001 Full text @ J. Cell Biol.
Citation
Etard, C., Roostalu, U., and Strähle, U. (2010) Lack of Apobec2-related proteins causes a dystrophic muscle phenotype in zebrafish embryos. The Journal of cell biology. 189(3):527-539.
Abstract
The chaperones Unc45b and Hsp90a are essential for folding of myosin in organisms ranging from worms to humans. We show here that zebrafish Unc45b, but not Hsp90a, binds to the putative cytidine deaminase Apobec2 (Apo2) in an interaction that requires the Unc45/Cro1p/She4p-related (UCS) and central domains of Unc45b. Morpholino oligonucleotide-mediated knockdown of the two related proteins Apo2a and Apo2b causes a dystrophic phenotype in the zebrafish skeletal musculature and impairs heart function. These phenotypic traits are shared with mutants of unc45b, but not with hsp90a mutants. Apo2a and -2b act nonredundantly and bind to each other in vitro, which suggests a heteromeric functional complex. Our results demonstrate that Unc45b and Apo2 proteins act in a Hsp90a-independent pathway that is required for integrity of the myosepta and myofiber attachment. Because the only known function of Unc45b is that of a chaperone, Apo2 proteins may be clients of Unc45b but other yet unidentified processes cannot be excluded.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping