PUBLICATION

Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube

Authors
Alexandre, P., Reugels, A.M., Barker, D., Blanc, E., and Clarke, J.D.
ID
ZDB-PUB-100511-25
Date
2010
Source
Nature Neuroscience   13(6): 673-679 (Journal)
Registered Authors
Alexandre, Paula, Barker, David, Clarke, Jon, Reugels, Alexander
Keywords
none
MeSH Terms
  • Animals
  • Rhombencephalon/anatomy & histology
  • Rhombencephalon/embryology
  • Rhombencephalon/physiology
  • Cell Division/physiology
  • Protein Kinase C/metabolism
  • Stem Cells/physiology*
  • Neurons/physiology*
  • Immunohistochemistry
  • Neurogenesis/physiology*
  • Neural Tube/anatomy & histology
  • Neural Tube/embryology*
  • Neural Tube/physiology*
  • Zebrafish
  • Time Factors
  • Microscopy, Confocal/methods
  • Video Recording
(all 17)
PubMed
20453852 Full text @ Nat. Neurosci.
Abstract
In the developing CNS, asymmetric cell division is critical for maintaining the balanced production of differentiating neurons while renewing the population of neural progenitors. In invertebrates, this process depends on asymmetric inheritance of fate determinants during progenitor divisions. A similar mechanism is widely believed to underlie asymmetrically fated divisions in vertebrates, but compelling evidence for this is missing. We used live imaging of individual progenitors in the intact zebrafish embryo CNS to test this hypothesis. We found that asymmetric inheritance of a subcellular domain is strongly correlated with asymmetric daughter fates and our results reveal an unexpected feature of this process. The daughter cell destined to become a neuron was derived from the more apical of the two daughters, whereas the more basal daughter inherited the basal process and replenished the apical progenitor pool.
Genes / Markers
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
knu3TgTransgenic Insertion
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    Human Disease / Model
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    Sequence Targeting Reagents
    Target Reagent Reagent Type
    pard3abMO1-pard3abMRPHLNO
    prkciMO1-prkciMRPHLNO
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    Orthology
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
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    Citation
    Alexandre, P., Reugels, A.M., Barker, D., Blanc, E., and Clarke, J.D. (2010) Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube. Nature Neuroscience. 13(6):673-679.