ZFIN ID: ZDB-PUB-100504-3
Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function
Mallappa, C., Nasipak, B.T., Etheridge, L., Androphy, E.J., Jones, S.N., Sagerström, C.G., Ohkawa, Y., and Imbalzano, A.N.
Date: 2010
Source: Molecular and cellular biology   30(13): 3176-3186 (Journal)
Registered Authors: Etheridge, Letitiah, Sagerström, Charles
Keywords: none
MeSH Terms:
  • Adaptor Proteins, Signal Transducing
  • Adenosine Triphosphate/metabolism*
  • Animals
  • Cells, Cultured
  • Chromatin/metabolism*
  • Chromatin Assembly and Disassembly*
  • DNA Helicases/genetics
  • DNA Helicases/metabolism*
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Muscle Development/genetics*
  • Myoblasts/cytology
  • Myoblasts/physiology
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Ribonuclease III/genetics
  • Ribonuclease III/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 20421421 Full text @ Mol. Cell. Biol.
Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies loss of the microRNA processing enzyme, Dicer, or knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. Binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.