PUBLICATION

Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Authors
Mallappa, C., Nasipak, B.T., Etheridge, L., Androphy, E.J., Jones, S.N., Sagerström, C.G., Ohkawa, Y., and Imbalzano, A.N.
ID
ZDB-PUB-100504-3
Date
2010
Source
Molecular and cellular biology   30(13): 3176-3186 (Journal)
Registered Authors
Etheridge, Letitiah, Sagerström, Charles
Keywords
none
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Adenosine Triphosphate/metabolism*
  • Animals
  • Cells, Cultured
  • Chromatin/metabolism*
  • Chromatin Assembly and Disassembly*
  • DNA Helicases/genetics
  • DNA Helicases/metabolism*
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Muscle Development/genetics*
  • Myoblasts/cytology
  • Myoblasts/physiology
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Ribonuclease III/genetics
  • Ribonuclease III/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
20421421 Full text @ Mol. Cell. Biol.
Abstract
Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies loss of the microRNA processing enzyme, Dicer, or knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. Binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes