PUBLICATION

The polarity protein Pard3 is required for centrosome positioning during neurulation

Authors
Hong, E., Jayachandran, P., and Brewster, R.
ID
ZDB-PUB-100211-20
Date
2010
Source
Developmental Biology   341(2): 335-345 (Journal)
Registered Authors
Brewster, Rachel, Hong, Elim
Keywords
none
MeSH Terms
  • Centrosome/metabolism*
  • Carrier Proteins/analysis
  • Carrier Proteins/metabolism*
  • Animals
  • Microtubules/metabolism*
  • Axoneme/metabolism
  • Cell Polarity*
  • Cilia/metabolism
  • Zebrafish/embryology*
  • Zebrafish Proteins/analysis
  • Zebrafish Proteins/metabolism*
  • Neurulation*
(all 12)
PubMed
20138861 Full text @ Dev. Biol.
Abstract
Microtubules are essential regulators of cell polarity, architecture and motility. The organization of the microtubule network is context-specific. In non-polarized cells, microtubules are anchored to the centrosome and form radial arrays. In most epithelial cells, microtubules are noncentrosomal, align along the apico-basal axis and the centrosome templates a cilium. It follows that cells undergoing mesenchyme-to-epithelium transitions must reorganize their microtubule network extensively, yet little is understood about how this process is orchestrated. In particular, the pathways regulating the apical positioning of the centrosome are unknown, a central question given the role of cilia in fluid propulsion, sensation and signaling. In zebrafish, neural progenitors undergo progressive epithelialization during neurulation, and thus provide a convenient in vivo cellular context in which to address this question. We demonstrate here that the microtubule cytoskeleton gradually transitions from a radial to linear organization during neurulation and that microtubules function in conjunction with the polarity protein Pard3 to mediate centrosome positioning. Pard3 depletion results in hydrocephalus, a defect often associated with abnormal cerebrospinal fluid flow that has been linked to cilia defects. These findings thus bring to focus cellular events occurring during neurulation and reveal novel molecular mechanisms implicated in centrosome positioning.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-pard3abstandard conditions5-9 somites
AB + MO1-pard3abstandard conditions10-13 somites
AB + MO1-pard3abstandard conditions10-13 somites
AB + MO1-pard3abstandard conditionsPrim-5
AB + MO1-pard3abstandard conditionsLong-pec
lgny14/y14 (TL)standard conditions1-4 somites to 5-9 somites
lgny14/y14 (TL)standard conditions1-4 somites to 5-9 somites
1 - 7 of 7
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
y14
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    pard3abMO1-pard3abMRPHLNO
    pard3abMO5-pard3abMRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    AB + MO1-pard3ab
    lgny14/y14 (TL)
    1 - 2 of 2
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Hong, E., Jayachandran, P., and Brewster, R. (2010) The polarity protein Pard3 is required for centrosome positioning during neurulation. Developmental Biology. 341(2):335-345.