PUBLICATION

Lgl2 executes its function as a tumor suppressor by regulating ErbB signaling in the zebrafish epidermis

Authors
Reischauer, S., Levesque, M.P., Nüsslein-Volhard, C., and Sonawane, M.
ID
ZDB-PUB-091120-51
Date
2009
Source
PLoS Genetics   5(11): e1000720 (Journal)
Registered Authors
Levesque, Mitch, Nüsslein-Volhard, Christiane, Reischauer, Sven, Sonawane, Mahendra
Keywords
Larvae, Phenotypes, Zebrafish, Cell polarity, Epidermis, Tumor suppressor genes, Signal inhibition, Microarrays
MeSH Terms
  • Animals
  • Cell Differentiation
  • Cell Proliferation
  • Cell Shape
  • Epidermis/growth & development*
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • Larva
  • Mesoderm
  • Mutation
  • Oncogene Proteins v-erbB/metabolism*
  • Phylogeny
  • Signal Transduction
  • Tumor Suppressor Proteins/genetics
  • Tumor Suppressor Proteins/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
19911055 Full text @ PLoS Genet.
Abstract
Changes in tissue homeostasis, acquisition of invasive cell characteristics, and tumor formation can often be linked to the loss of epithelial cell polarity. In carcinogenesis, the grade of neoplasia correlates with impaired cell polarity. In Drosophila, lethal giant larvae (lgl), discs large (dlg), and scribble, which are components of the epithelial apico-basal cell polarity machinery, act as tumor suppressors, and orthologs of this evolutionary conserved pathway are lost in human carcinoma with high frequency. However, a mechanistic link between neoplasia and vertebrate orthologs of these tumor-suppressor genes remains to be fully explored at the organismal level. Here, we show that the pen/lgl2 mutant phenotype shares two key cellular and molecular features of mammalian malignancy: cell autonomous epidermal neoplasia and epithelial-to-mesenchymal-transition (EMT) of basal epidermal cells including the differential expression of several regulators of EMT. Further, we found that epidermal neoplasia and EMT in pen/lgl2 mutant epidermal cells is promoted by ErbB signalling, a pathway of high significance in human carcinomas. Intriguingly, EMT in the pen/lgl2 mutant is facilitated specifically by ErbB2 mediated E-cadherin mislocalization and not via canonical snail-dependent down-regulation of E-cadherin expression. Our data reveal that pen/lgl2 functions as a tumor suppressor gene in vertebrates, establishing zebrafish pen/lgl2 mutants as a valuable cancer model.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes