ZFIN ID: ZDB-PUB-091120-34
Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter
Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L.
Date: 2010
Source: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   155(2): 155-163 (Journal)
Registered Authors: Chen, Jyh-Yih, Wu, Jen-Leih
Keywords: Follicle-stimulating hormone gene, Promoter analysis, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Bioreactors
  • Calcium/pharmacology
  • Cell Line
  • Cloning, Molecular
  • Embryo, Nonmammalian/metabolism
  • Enzyme Activation
  • Female
  • Fertilization
  • Follicle Stimulating Hormone, beta Subunit/genetics*
  • Follicle Stimulating Hormone, beta Subunit/metabolism
  • Gene Expression Regulation/drug effects
  • Gonadotropin-Releasing Hormone/metabolism
  • Mitogen-Activated Protein Kinase 1/metabolism
  • Mitogen-Activated Protein Kinase 3/metabolism
  • Ovary/cytology
  • Ovary/drug effects
  • Ovary/metabolism
  • Ovum/physiology
  • Pituitary Gland/metabolism
  • Promoter Regions, Genetic/genetics*
  • Protein Kinase C/metabolism
  • Signal Transduction
  • Time Factors
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/physiology
PubMed: 19896554 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
ABSTRACT
In the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSHbeta promoter responded to 6h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSHbeta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green fluorescent protein (GFP) fragments encoding the GFP complementary DNA transgene which was microinjected into zebrafish embryos. Morphological studies of transgenic zebrafish indicated that the FSHbeta promoter-driven GFP transcripts appeared in the heart, skin, and vertebrae.
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