|ZFIN ID: ZDB-PUB-091120-34|
Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter
Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L.
|Source:||Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 155(2): 155-163 (Journal)|
|Registered Authors:||Chen, Jyh-Yih, Wu, Jen-Leih|
|Keywords:||Follicle-stimulating hormone gene, Promoter analysis, Zebrafish|
|PubMed:||19896554 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.|
Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L. (2010) Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 155(2):155-163.
ABSTRACTIn the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSHbeta promoter responded to 6h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSHbeta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green fluorescent protein (GFP) fragments encoding the GFP complementary DNA transgene which was microinjected into zebrafish embryos. Morphological studies of transgenic zebrafish indicated that the FSHbeta promoter-driven GFP transcripts appeared in the heart, skin, and vertebrae.
- Genes / Markers (1)