PUBLICATION
The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella
- Authors
- Xu, Q.Q., Chang, M.X., Xiao, F.S., Huang, B., and Nie, P.
- ID
- ZDB-PUB-091120-31
- Date
- 2010
- Source
- Veterinary Immunology and Immunopathology 134(3-4): 269-278 (Journal)
- Registered Authors
- Nie, Pin
- Keywords
- IRF-5, Genome, Expression, GCHV, Grass carp, Ctenopharyngodon idella
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/genetics*
- Carps/immunology*
- Cloning, Molecular
- DNA Primers/genetics
- DNA, Complementary/genetics
- Fish Diseases/genetics
- Fish Diseases/immunology
- Fish Diseases/virology
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/immunology*
- Gene Expression
- Immunity, Innate
- Interferon Regulatory Factors/chemistry
- Interferon Regulatory Factors/genetics*
- Interferon Regulatory Factors/immunology*
- Kidney/immunology
- Molecular Sequence Data
- Open Reading Frames
- Phylogeny
- Protein Structure, Tertiary
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reoviridae/immunology
- Reoviridae/pathogenicity
- Reoviridae Infections/genetics
- Reoviridae Infections/immunology
- Reoviridae Infections/veterinary
- Sequence Homology, Amino Acid
- Spleen/immunology
- PubMed
- 19896215 Full text @ Vet. Immunol. Immunopathol.
Citation
Xu, Q.Q., Chang, M.X., Xiao, F.S., Huang, B., and Nie, P. (2010) The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella. Veterinary Immunology and Immunopathology. 134(3-4):269-278.
Abstract
The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenopharyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping