Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gammarel) in grass carp Ctenopharyngodon idella
- Chen, W.Q., Xu, Q.Q., Chang, M.X., Zou, J., Secombes, C.J., Peng, K.M., and Nie, P.
- Veterinary Immunology and Immunopathology 134(3-4): 199-207 (Journal)
- Registered Authors
- Nie, Pin
- Grass carp, IFN-γ related gene, Reovirus, Peptidoglycan, Nucleotide oligomerization domain
- MeSH Terms
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Primers/genetics
- DNA, Complementary/genetics
- Fish Diseases/genetics
- Fish Diseases/immunology
- Fish Proteins/genetics*
- Gene Expression
- Molecular Sequence Data
- Reoviridae Infections/genetics
- Reoviridae Infections/immunology
- Reoviridae Infections/veterinary
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- 19800136 Full text @ Vet. Immunol. Immunopathol.
Chen, W.Q., Xu, Q.Q., Chang, M.X., Zou, J., Secombes, C.J., Peng, K.M., and Nie, P. (2010) Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gammarel) in grass carp Ctenopharyngodon idella. Veterinary Immunology and Immunopathology. 134(3-4):199-207.
Interferon gamma (IFN-gamma), the only member of the type II class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gammarel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gammarel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gammarel (gcIFN-gammarel) showed 63% and 50% identities to zebrafish and common carp IFN-gammarel (previously termed as IFN-gamma1), respectively. The IFN-gammarel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2kb of genomic sequence. The gcIFN-gammarel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gammarel in spleen. The expression of gcIFN-gammarel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gammarel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gammarel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gammarel was possibly activated in a NOD2-dependent mechanism.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes