PUBLICATION

Chromosomal position mediates spinal cord expression of a dbx1a enhancer

Authors
Gribble, S.L., Nikolaus, O.B., Carver, M.S., Hoshijima, K., and Dorsky, R.I.
ID
ZDB-PUB-091023-65
Date
2009
Source
Developmental dynamics : an official publication of the American Association of Anatomists   238(11): 2929-2935 (Journal)
Registered Authors
Dorsky, Richard, Gribble, Suzanna L., Hoshijima, Kazuyuki
Keywords
dbx1a enhancer, expression, spinal cord, transgene expression, zebrafish
MeSH Terms
  • Animals
  • Chromosomes
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology*
  • Embryo, Nonmammalian/metabolism
  • Enhancer Elements, Genetic/genetics*
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter/genetics
  • Genes, Reporter/physiology
  • Homeodomain Proteins/genetics*
  • Promoter Regions, Genetic/genetics
  • Promoter Regions, Genetic/physiology
  • Spinal Cord/embryology*
  • Spinal Cord/metabolism
  • Transcription Factors/genetics*
  • Transgenes/genetics
  • Transgenes/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
PubMed
19842185 Full text @ Dev. Dyn.
Abstract
Dbx homeodomain proteins are important for the production of multiple spinal cord cell types. To examine the regulation of Dbx genes in more detail, we have generated transgenic zebrafish in which fluorescent protein expression is driven by predicted dbx1a enhancers. We identified three areas of sequence conservation upstream of the dbx1a coding sequence and generated fluorescent reporter constructs driven by these predicted enhancer elements and the endogenous dbx1a promoter. In multiple stable insertions of a 3.5-kb enhancer fragment, we observed that there was additional reporter expression in the dorsal spinal cord not normally observed by dbx1a in situ hybridization. In addition, these lines exhibited only transient reporter expression, unlike the endogenous gene. Surprisingly, a single insertion line expressed the reporter in the endogenous pattern, indicating that other local regulatory elements modulate gene expression through the 3.5-kb enhancer.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping