PUBLICATION
yap is required for the development of brain, eyes, and neural crest in zebrafish
- Authors
- Jiang, Q., Liu, D., Gong, Y., Wang, Y., Sun, S., Gui, Y., and Song, H.
- ID
- ZDB-PUB-090429-7
- Date
- 2009
- Source
- Biochemical and Biophysical Research Communications 384(1): 114-119 (Journal)
- Registered Authors
- Jiang, Qiu, Song, Houyan, Wang, Yuexiang
- Keywords
- yap, Brain, Neural progenitor cells, Eyes, Branchial arches, Neural crest, Apoptosis, Zebrafish
- MeSH Terms
-
- Animals
- Brain/embryology*
- Carrier Proteins/genetics
- Carrier Proteins/physiology*
- Eye/embryology*
- Humans
- Mice
- Neural Crest/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 19393221 Full text @ Biochem. Biophys. Res. Commun.
Citation
Jiang, Q., Liu, D., Gong, Y., Wang, Y., Sun, S., Gui, Y., and Song, H. (2009) yap is required for the development of brain, eyes, and neural crest in zebrafish. Biochemical and Biophysical Research Communications. 384(1):114-119.
Abstract
The Yes-associated protein (YAP) is a small protein that binds to many transcription factors and modulates their activity. Bioinformatics analysis indicated that zebrafish Yap shares high identity with its orthologs in fruit fly, chicken, mouse, and human. Expression analysis revealed that maternal transcripts of yap are ubiquitous, and endogenous yap is chronologically expressed in the notochord, brain, eyes, branchial arches, and pectoral fins. Knockdown of yap causes distinct morphological defects in embryos, which display a small head with smaller eyes than normal and fewer cartilages in the branchial arches. Proneural and neuronal gene expression in yap morphant brain is significantly reduced. The expression of crestin is also markedly reduced in all recognizable arch-associated regions of yap morphants. Furthermore, TUNEL analysis revealed that there is a marked increase in cell death in yap morphant brain. In conclusion, zebrafish yap is required for the development of the brain, eyes, and neural crest during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping