PUBLICATION
Highly Efficient ENU Mutagenesis in Zebrafish
- Authors
- de Bruijn, E., Cuppen, E., and Feitsma, H.
- ID
- ZDB-PUB-090422-21
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 3-12 (Chapter)
- Registered Authors
- Cuppen, Edwin, de Bruijn, Ewart, Feitsma, Harma
- Keywords
- Zebrafish, ENU, Mutagenesis, TILLING, Method, Mutant, Knockout
- MeSH Terms
-
- Ethylnitrosourea/administration & dosage*
- Zebrafish/genetics*
- Alkylating Agents/administration & dosage
- Animals
- Female
- Genetic Engineering/methods
- Animals, Genetically Modified
- Drug Administration Schedule
- Genome/drug effects
- DNA/drug effects
- Male
- Germ-Line Mutation
- Mutagenesis/drug effects*
- PubMed
- 19378094 Full text @ Meth. Mol. Biol.
Citation
de Bruijn, E., Cuppen, E., and Feitsma, H. (2009) Highly Efficient ENU Mutagenesis in Zebrafish. Methods in molecular biology (Clifton, N.J.). 546:3-12.
Abstract
ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping