PUBLICATION

Highly Efficient ENU Mutagenesis in Zebrafish

Authors
de Bruijn, E., Cuppen, E., and Feitsma, H.
ID
ZDB-PUB-090422-21
Date
2009
Source
Methods in molecular biology (Clifton, N.J.)   546: 3-12 (Chapter)
Registered Authors
Cuppen, Edwin, de Bruijn, Ewart, Feitsma, Harma
Keywords
Zebrafish, ENU, Mutagenesis, TILLING, Method, Mutant, Knockout
MeSH Terms
  • Alkylating Agents/administration & dosage
  • Animals
  • Animals, Genetically Modified
  • DNA/drug effects
  • Drug Administration Schedule
  • Ethylnitrosourea/administration & dosage*
  • Female
  • Genetic Engineering/methods
  • Genome/drug effects
  • Germ-Line Mutation
  • Male
  • Mutagenesis/drug effects*
  • Zebrafish/genetics*
PubMed
19378094 Full text @ Meth. Mol. Biol.
Abstract
ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping