ZFIN ID: ZDB-PUB-090417-13
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y.
Date: 2009
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience   29(15): 4756-4767 (Journal)
Registered Authors: Higashijima, Shin-ichi, Miyasaka, Nobuhiko, Morimoto, Kozo, Okamoto, Hitoshi, Yoshihara, Yoshihiro
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental/physiology*
  • Olfactory Bulb/embryology
  • Olfactory Bulb/physiology*
  • Olfactory Pathways/embryology
  • Olfactory Pathways/physiology*
  • Olfactory Receptor Neurons/embryology
  • Olfactory Receptor Neurons/physiology
  • Zebrafish
PubMed: 19369545 Full text @ J. Neurosci.
In the vertebrate olfactory system, odor information is represented as a topographic map in the olfactory bulb (OB). However, it remains unknown how this odor map is transferred from the OB to higher olfactory centers. Using genetic labeling techniques in zebrafish, we found that the OB output neurons, mitral cells (MCs), are heterogeneous with respect to transgene expression profiles and spatial distributions. Tracing MC axons at single-cell resolution revealed that (1) individual MCs send axons to multiple target regions in the forebrain; (2) MCs innervating the same glomerulus do not necessarily display the same axon trajectory; (3) MCs innervating distinct glomerular clusters tend to project axons to different, but partly overlapping, target regions; (4) MCs innervating the medial glomerular cluster directly and asymmetrically send axons to the right habenula. We propose that the topographic odor map in the OB is not maintained intact, but reorganized in higher olfactory centers. Moreover, our finding of asymmetric bulbo-habenular projection renders the olfactory system an attractive model for the studies of brain asymmetry and lateralized behaviors.