PUBLICATION
Large-scale small-molecule screen using zebrafish embryos
- Authors
- Hong, C.C.
- ID
- ZDB-PUB-090407-23
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 486: 43-55 (Chapter)
- Registered Authors
- Hong, Charles
- Keywords
- Chemical genetics, Chemical screen, Drug discovery, Large scale, Phenotype, Small-molecule library, Zebrafish
- MeSH Terms
-
- Animals
- Drug Evaluation, Preclinical/methods*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Small Molecule Libraries/pharmacology*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 19347615 Full text @ Meth. Mol. Biol.
Citation
Hong, C.C. (2009) Large-scale small-molecule screen using zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 486:43-55.
Abstract
Zebrafish represents a versatile model organism with many molecular, morphological, and physiological similarities to mammals. Importantly, zebrafish are readily susceptible to perturbations by small molecules, including numerous pharmaceuticals in clinical use. Given these qualities, plus their small size and transparency, zebrafish embryos can be utilized for large-scale phenotype-based screens for small-molecule modifiers of biological processes. Thus, in a manner analogous to classical genetic screens, zebrafish chemical screens have the potential to reveal novel insights into complex biological pathways, as well as to identify lead compounds for novel therapeutics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping