ZFIN Person: Hong, Charles

ZFIN ID: ZDB-PERS-030925-5

ZFIN ID: ZDB-PERS-030925-5

Hong, Charles

Email:	Charles.hong@som.umaryland.edu
URL:	https://www.medschool.umaryland.edu/profiles/Hong-Charles/
Affiliation:	Hong Lab
Address:	Charles C. Hong, MD, PhD University of Maryland School of Medicine Health Sciences Facility III, 7110 (lab) 670 West Baltimore Street Baltimore, MD 21201 USA
Country:
Phone:	410-706-2681
Fax:
ORCID ID:	0000-0092-0424-8252

BIOGRAPHY AND RESEARCH INTERESTS

I grew up in Detroit, then spent 20 plus years in East Coast. I got my PhD in Drosophila embryonic axis formation at Yale with Carl Hashimoto, then did postdoc in chemical biology with Randy Peterson at Harvard. After 12 wonderful years at Vanderbilt, I moved to U of Maryland School of Medicine to serve as Co-Chief of Cardiovascular Medicine and direct its cardiology research.

PUBLICATIONS

Ng, V.H., Spencer, Z., Neitzel, L.R., Nayak, A., Loberg, M.A., Shen, C., Kassel, S.N., Kroh, H.K., An, Z., Anthony, C.C., Bryant, J.M., Lawson, A., Goldsmith, L., Benchabane, H., Hansen, A.G., Li, J., D'Souza, S., Lebensohn, A.M., Rohatgi, R., Weiss, W.A., Weiss, V.L., Williams, C., Hong, C.C., Robbins, D.J., Ahmed, Y., Lee, E. (2023) The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling. Nature communications. 14:61736173

Shen, C., Nayak, A., Neitzel, L.R., Adams, A.A., Silver-Isenstadt, M., Sawyer, L.M., Benchabane, H., Wang, H., Bunnag, N., Li, B., Wynn, D.T., Yang, F., Garcia-Contreras, M., Williams, C.H., Dakshanamurthy, S., Hong, C.C., Ayad, N.G., Capobianco, A.J., Ahmed, Y., Lee, E., Robbins, D.J. (2021) The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling. Nature communications. 12:5263

Williams, C.H., Hong, C.C. (2016) Zebrafish small molecule screens: Taking the phenotypic plunge. Computational and structural biotechnology journal. 14:350-356

Hempel, J.E., Cadar, A.G., Hong, C.C. (2016) Development of thieno- and benzopyrimidinone inhibitors of the Hedgehog signaling pathway reveals PDE4-dependent and PDE4-independent mechanisms of action. Bioorganic & medicinal chemistry letters. 26(8):1947-53

Williams, C.H., Hempel, J.E., Hao, J., Frist, A.Y., Williams, M.M., Fleming, J.T., Sulikowski, G.A., Cooper, M.K., Chiang, C., Hong, C.C. (2015) An In Vivo Chemical Genetic Screen Identifies Phosphodiesterase 4 as a Pharmacological Target for Hedgehog Signaling Inhibition. Cell Reports. 11(1):43-50

Williams, C.H., Hong, C.C. (2015) High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1263:167-174

Hao, J., Ao, A., Zhou, L., Murphy, C.K., Frist, A.Y., Keel, J.J., Thorne, C.A., Kim, K., Lee, E., and Hong, C.C. (2013) Selective Small Molecule Targeting β-Catenin Function Discovered by In Vivo Chemical Genetic Screen. Cell Reports. 4(5):898-904

Wiley, D.M., Kim, J.D., Hao, J., Hong, C.C., Bautch, V.L., and Jin, S.W. (2011) Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein. Nature cell biology. 13(6):686-92

Williams, C.H., and Hong, C.C. (2011) Multi-step usage of in vivo models during rational drug design and discovery. International Journal of Molecular Sciences. 12(4):2262-2274

Hao, J., Williams, C.H., Webb, M.E., and Hong, C.C. (2010) Large Scale Zebrafish-Based In vivo Small Molecule Screen. Journal of visualized experiments : JoVE. (46)

Hao, J., Ho, J.N., Lewis, J.A., Karim, K.A., Daniels, R.N., Gentry, P.R., Hopkins, C., Lindsley, C., and Hong, C.C. (2010) In Vivo Structure Activity Relationship Study of Dorsomorphin Analogs Identifies Selective VEGF and BMP Inhibitors. ACS Chemical Biology. 5(2):245-253

Hong, C.C. (2009) Large-scale small-molecule screen using zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 486:43-55

Hong, C.C., Kume, T., and Peterson, R.T. (2008) Role of crosstalk between phosphatidylinositol 3-kinase and extracellular signal-regulated kinase/mitogen-activated protein kinase pathways in artery-vein specification. Circulation research. 103(6):573-579

Yu, P.B., Hong, C.C., Sachidanandan, C., Babitt, J.L., Deng, D.Y., Hoyng, S.A., Lin, H.Y., Bloch, K.D., and Peterson, R.T. (2008) Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nature Chemical Biology. 4(1):33-41

Hong, C.C., Peterson, Q.P., Hong, J.Y., and Peterson, R.T. (2006) Artery/vein specification is governed by opposing phosphatidylinositol-3 kinase and MAP kinase/ERK signaling. Current biology : CB. 16(13):1366-1372

NON-ZEBRAFISH PUBLICATIONS
Wells QS, Becker JR, Su YR, Mosley JD, Weeke P, D’Aoust L, Ausborn NL, Ramirez AH, Pfotenhauer JP, Naftilan A, Markham L, Exil V, Roden DM, Hong CC. Whole exome sequencing identifies a causal RBM20 mutation in a large pedigree with familial dilated cardiomyopathy. Circulation Cardiovascular Genetics 2013; 6:317-326.

Roden DM, Hong CC. Stem Cell-Derived Cardiomyocytes as a Tool for Studying Proarrhythmia: A Better Canary in the Coal Mine? Circulation 2013; 127:1641-3.

Shelton EL, Galindo CL, Williams CH, Pfaltzgraff E, Hong CC, Bader DM. Autotaxin signaling governs phenotypic heterogeneity in visceral and parietal mesothelia. PLoS ONE 2013; 8:e69712.

Williams CH, Hong CC. Making models work: library annotation through phenoclustering. Drug Discovery Today – Model Organisms 2013; 10:e5-e9.

Owens P, Polikowsky H, Pickup MW, Gorska AE, Javanovic B, Shaw AK, Nivitskiy SV, Hong CC, Moses HL. Bone morphogenetic proteins stimulate mammary fibroblasts to promote mammary carcinoma cell invasion. PLoS ONE 2013; 8:e67533.

Langenfeld E, Hong CC, Lanke G, Langenfeld J. Bone morphogenetic protein type-I receptor antagonists decrease growth and induce cell death of lung cancer cell lines. PLoS ONE 2013; 8:e61256.

Engers, DW, Frist AY, Lindsley CW, Hong CC, Hopkins CR. Synthesis and structure-activity relationships of a novel and selective bone morphogenetic protein (BMP) receptor inhibitor derived from the pyrazolo[1,5-a]pyrimidine scaffold of Dorsomorphin: the discovery of ML347 as an ALK2 versus ALK3 selective MLPCN probe. Bioorganic & Medicinal Chemistry Letters 2013; 23:3248-52.

Sun CC, Vaja V, Chen S, Theurl I, Stephanek A, Brown D, Cappellini MD, Hong CC,
Lin HY, Babitt JL. A hepcidin inhibitor mobilizes iron for incorporation into red blood cells in an adenine-induced chronic kidney disease model in rats. Nephrology Dialysis Transplantation 2013; 28:1733-43.

Sheng CC, Hong CC. Mixing of the old with the new: nanoparticle-mediated pioglitazone delivery to enhance therapeutic neovascularization. Arteriosclerosis, Thrombosis, and Vascular Biology 2012; 10:2337-2338.

Ao A, Hao J, Hopkins CR, Hong CC. DMH1, a novel BMP small molecule inhibitor, increases cardiomyocyte progenitors and promotes cardiac differentiation in mouse embryonic stem cells PLoS ONE 2012; 7:e41627.

Neely MD, Litt, MJ, Aboud AA, Tidball AM, Li GG, Hedera P, Hong CC, Ess KC, Bowman AB. Differential pattern of neural precursor marker induction in hiPSCs neutralized with noggin or the dorsomorphin analogue, DMH1. ACS Chemical Neuroscience 2012; 3:482-491.

Hill CR, Sanchez NS, Love JD, Arrieta JA, Hong CC, Brown CB, Austin FB, Barnett JV. BMP2 signals loss of epithelial character in epicardial cells but requires the Type III TGFreceptor to promote invasion. Cell Signal 2012; 24:1012-1022.

Saeed O, Otsuka F, Polavarapu R, Karmali V, Weiss D, Davis T, Rostad B, Pachura K, Adams L, Elliott J, Taylor R, Narula J, Kolodgie F, Virmani R, Hong CC**, Finn AV**. Pharmacologic suppression of hepcidin increases macrophage cholesterol efflux and reduces foam cell formation and atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology 2012; 32:299-307. **co-corresponding authors.

Wang, L, Trebicka, E, Fu, Y, Ellenbogen, S, Hong, CC, Babitt, JL, Lin, HY, Cherayil, BJ. The bone morphogenetic protein-hepcidin axis as a therapeutic target in inflammatory bowel disease. Inflammatory Bowel Diseases 2012; 18:112-119.

Ao A, Hao J, Hong CC. Regenerative chemical biology: current challenges and future potentials. Chemistry & Biology 2011; 18:413-424.

Gupta MK, Crowder SW, Jung DK, Walthal JM, Venkataramn R, Yu SCS, Feaster TK, Hong CC, Baudenbacher FJ, Hatzopoulos AK, Sung HJ. Combinatorial polymer electrospun matrices promote physiologically-relevant cardiomyogenic stem cell differentiation. PLoS ONE 2011; 6:e28935.

Wells QS, Ausborn NL, Funke BH, Pfotenhouer JP, Fredi JL, Thomas D. DiSalvo TD, Hong CC. A familial dilated cardiomyopathy associated with a novel VCL mutation (Lys815Arg) in conjunction with a known MYPBC3 variant: possible gene-gene and genotype-phenotype interactions. Cardiogenetics 2011; 1:e10.

Cross EE, Martinez M, Thomason RT, Hokpins, CR, Hong CC*, Bader DM*. Application of Small Organic Molecules Reveals Cooperative TGFβ and BMP Regulation of Mesothelial Cell Behaviors. ACS Chemical Biology 2011; 6:952-961. **co-corresponding authors.

Theurl I, Schroll A, Sonnweber T, Nairz M, Theurl M, Willenbacher W, Eller K, Wolf D, Seifert M, Sun CC, Babitt JL, Hong CC, Menhall T, Gearing P, Lin HY, Weiss G. Pharmacologic inhibition of hepcidin reverses anemia of chronic disease in rats. Blood 2011; 118:4977-4984.

Palmisano BT, Rottman JN, DiSalvo TG, Hong CC. Familial evaluation for diagnosis of arrhythmogenic right ventricular dysplasia. Cardiology 2011; 119:47-53.

Ao A, Williams CH, Hao J, Hong CC. Modified mouse embryonic stem cell based assay for quantifying cardiogenic induction efficiency. JoVE 2011; pii:2656.

Xia Y, Cortez-Retamozo V, Niederkofler V, Salie R, Chen S, Samad T, Hong CC, Arber S, Vyas JM, Weissleder R, Pittet MJ, Lin HY. Dragon (RGMb) inhibits IL-6 expression in macrophages. J. Immunology 2011; 187:1369-1376.

Shi ST, Hoogaars WMH, de Gorter DJJ, van Heiningen SH, Lin HY, Hong CC, Kemaladewi DU, Aartsma-Rus A, ten Dijke P, ‘T Hoen PAC. BMP antagonists enhance myogenic differentiation and ameliorate the dystrophic phenotype in a DMD mouse model. Neurobiology of Disease 2011; 41:353-360.

Wang H, Hao J, Hong CC. Cardiac induction of embryonic stem cells by a small molecule inhibitor of Wnt/-catenin signaling. ACS Chemical Biology 2011; 6:192-197.

Xia T, Babitt JL, Bouley R, Zhang Y, Da Silva N, Chen S, Zhuang Z, Samad TA, Brenner GJ, Anderson JL, Hong CC, Schneyer AL, Brown D, Lin HY. Dragon mediates BMP signaling and increases transepithelial resistance in kidney epithelial cells. Journal of the American Society of Nephrology 2010; 21:666-677.

Hao J, Daleo MA, Hong CC. Crosstalk between mitogen-activated protein kinase and phosphoinositide-3 kinase signaling in development and disease. In: Systems Biology for Signaling Networks. Choi S, ed. New York, NY, Springer. 2010; pp. 505-530.

Kaplan FS, Zasloff MA, Kitterman JA, Shore EM, Hong CC, Rocke D. Early mortality and cardiorespiratory failure in patients with fibrodysplasia ossificans progressiva. Journal of Bone and Joint Surgery 2009; 92:686-691.

Hong, CC, Yu, PB. Applications of small molecule BMP inhibitors in physiology and disease. Cytokine Growth Factor Rev, 20(5-6), 409-18, 2009

Wang, L, Harrington, L, Trebicka, E, Shi, HN, Kagan, JC, Hong, CC, Lin, HY, Babitt, JL, Cherayil, BJ. Selective modulation of TLR4-activated inflammatory responses by altered iron homeostasis in mice. J Clin Invest, 119(11), 3322-8, 2009.

Hao, J, Daleo, MA, Murphy, CK, Yu, PB, Ho, JN, Hu, J, Peterson, RT, Hatzopoulos, AK, Hong, CC. Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS ONE, 3(8), e2904, 2008.

Yu, PB, Deng, DY, Beppu, H, Hong, CC, Lai, C, Hoyng, SA, Kawai, N, Bloch, KD. Bone morphogenetic protein (BMP) type II receptor is required for BMP-mediated growth arrest and differentiation in pulmonary artery smooth muscle cells. J Biol Chem, 283(7), 3877-88, 2008

Yu, PB, Deng, DY, Lai, CS, Hong, CC, Cuny, GD, Bouxsein, ML, Hong, DW, McManus, PM, Katagiri, T, Sachidanandan, C, Kamiya, N, Fukuda, T, Mishina, Y, Peterson, RT, Bloch, KD. BMP type I receptor inhibition reduces heterotopic ossification. Nat Med, 14(12), 1363-9, 2008