PUBLICATION
olig1 expression identifies developing oligodendrocytes in zebrafish and requires hedgehog and notch signaling
- Authors
- Schebesta, M., and Serluca, F.C.
- ID
- ZDB-PUB-090309-10
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(4): 887-898 (Journal)
- Registered Authors
- Serluca, Fabrizio
- Keywords
- olig1, olig2, myelin, oligodendrocytes, zebrafish, hedgehog, notch, eGFP
- MeSH Terms
-
- Brain/cytology
- Brain/embryology
- Brain/metabolism
- Gene Expression Regulation, Developmental
- Animals, Genetically Modified
- PubMed
- 19253391 Full text @ Dev. Dyn.
Abstract
Myelin, the isolating sheath around large diameter axons, is formed in the central nervous system (CNS) by oligodendrocytes. We isolated the zebrafish ortholog of olig1, a bHLH transcription factor, and describe the origin and development of oligodendrocytes in the zebrafish brain. Olig1:mem-eGFP transgenic animals demonstrate the highly dynamic nature of oligodendrocyte membrane processes, providing a tool for studying in vivo oligodendrocyte development. Formation of oligodendrocytes and initiation of olig1 expression are under the control of long-range hedgehog and notch signaling while maintenance of olig1 expression only depends on hedgehog. Over-expression of olig1 did not affect myelin formation in the brain and combined over-expression of olig1 and olig2 could not rescue loss of hedgehog signaling, indicating that critical factors other than olig1 and olig2 are necessary. Lastly, knockdown of Olig1 in an Olig2-sensitized background did result in defects in CNS myelination, indicating a functional overlap between Olig1 and Olig2 proteins.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping