PUBLICATION
Automated image-based phenotypic analysis in zebrafish embryos
- Authors
- Vogt, A., Cholewinski, A., Shen, X., Nelson, S.G., Lazo, J.S., Tsang, M., and Hukriede, N.A.
- ID
- ZDB-PUB-090302-12
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(3): 656-663 (Journal)
- Registered Authors
- Hukriede, Neil
- Keywords
- cognition network technology, high-content screening, angiogenesis, pironetin, zebrafish
- MeSH Terms
-
- Angiogenesis Inhibitors/pharmacology
- Image Processing, Computer-Assisted/methods*
- Genes, Reporter/genetics
- Phenotype
- Animals
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Embryo, Nonmammalian/blood supply
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology*
- Embryo, Nonmammalian/metabolism*
- PubMed
- 19235725 Full text @ Dev. Dyn.
Citation
Vogt, A., Cholewinski, A., Shen, X., Nelson, S.G., Lazo, J.S., Tsang, M., and Hukriede, N.A. (2009) Automated image-based phenotypic analysis in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(3):656-663.
Abstract
Presently, the zebrafish is the only vertebrate model compatible with contemporary paradigms of drug discovery. Zebrafish embryos are amenable to automation necessary for high-throughput chemical screens, and optical transparency makes them potentially suited for image-based screening. However, the lack of tools for automated analysis of complex images presents an obstacle to using the zebrafish as a high-throughput screening model. We have developed an automated system for imaging and analyzing zebrafish embryos in multi-well plates regardless of embryo orientation and without user intervention. Images of fluorescent embryos were acquired on a high-content reader and analyzed using an artificial intelligence-based image analysis method termed Cognition Network Technology (CNT). CNT reliably detected transgenic fluorescent embryos (Tg(fli1:EGFP)(y1)) arrayed in 96-well plates and quantified intersegmental blood vessel development in embryos treated with small molecule inhibitors of anigiogenesis. The results demonstrate it is feasible to adapt image-based high-content screening methodology to measure complex whole organism phenotypes. Developmental Dynamics 238:656-663, 2009. (c) 2009 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping