PUBLICATION
Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment
- Authors
- Horng, J.L., Lin, L.Y., and Hwang, P.P.
- ID
- ZDB-PUB-090217-34
- Date
- 2009
- Source
- American journal of physiology. Cell physiology 296(4): C682-C692 (Journal)
- Registered Authors
- Horng, Jiun-Lin, Hwang, Pung Pung
- Keywords
- ionocytes, acid-base regulation, ion regulation, proliferation, differentiation
- MeSH Terms
-
- Acid-Base Equilibrium*
- Adaptation, Physiological
- Animals
- Apoptosis
- Cell Differentiation
- Cell Proliferation
- Embryonic Stem Cells/enzymology*
- Embryonic Stem Cells/ultrastructure
- Epithelial Cells/enzymology*
- Epithelial Cells/ultrastructure
- Fresh Water/chemistry
- Hydrogen-Ion Concentration
- Immunohistochemistry
- Microscopy, Confocal
- Microscopy, Electron, Scanning
- Phosphoproteins/metabolism
- Proliferating Cell Nuclear Antigen/metabolism
- Proton-Translocating ATPases/metabolism*
- Skin/embryology
- Skin/enzymology*
- Skin/ultrastructure
- Time Factors
- Trans-Activators/metabolism
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- PubMed
- 19211913 Full text @ Am. J. Physiol. Cell Physiol.
Citation
Horng, J.L., Lin, L.Y., and Hwang, P.P. (2009) Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment. American journal of physiology. Cell physiology. 296(4):C682-C692.
Abstract
It is important to maintain the internal pH homeostasis in biological systems. In our previous studies, H(+)-ATPase-rich (HR) cells were found to be responsible for proton secretion in the skin of zebrafish embryos during development. In this study, zebrafish embryos were exposed to acidic and basic waters to investigate the regulation of HR cell acid secretion during pH disturbances. Our results showed that the function of HR cells on the skin of zebrafish embryos can be upregulated in pH 4 water not only by increasing the cell number but also by enlarging the acid-secreting function of single cells. We also identified an "alveolar-type" apical opening under SEM observations of the apical membrane of HR cells, and the density and size of the alveolar type of apical openings also increased in pH 4 water. The p63 and PCNA immunostaining results also showed that additional HR cells in pH 4 water may be differentiated not only from ionocyte precursor cells but also newly proliferating epithelial stem cells. Key words: ionocytes, acid-base regulation, ion regulation, proliferation, differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping