Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways
- Chang, M.X., Wang, Y.P., and Nie, P.
- Fish & shellfish immunology 26(2): 264-274 (Journal)
- Registered Authors
- Chang, Mingxian, Nie, Pin
- zebrafish peptidoglycan recognition protein PGRP-SC (zfPGRP-SC), Danio rerio, RNA interference, microarray, signaling pathways, NF-κB
- MeSH Terms
- Carrier Proteins/immunology*
- Embryo, Nonmammalian/metabolism
- Gene Expression Profiling
- Gene Expression Regulation
- Gene Knockdown Techniques
- HeLa Cells
- Intracellular Space/physiology*
- NF-kappa B/immunology
- RNA, Small Interfering
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction*
- Toll-Like Receptors/immunology
- 19084604 Full text @ Fish Shellfish Immunol.
Chang, M.X., Wang, Y.P., and Nie, P. (2009) Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways. Fish & shellfish immunology. 26(2):264-274.
Insect PGRPs can function as bacterial recognition molecules triggering proteolytic and/or signal transduction pathways, with the resultant production of antimicrobial peptides. To explore if zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) has such effects, RNA interference (siRNA) and high-density oligonucleotide microarray analysis were used to identify differentially expressed genes regulated by zfPGRP-SC. The mRNA levels for a set of genes involved in Toll-like receptor signaling pathway, such as TLRs, SARM, MyD88, TRAF6 and nuclear factor (NF)-kappa B2 (p100/p52), were examined by quantitative RT-PCR (QT-PCR). The results from the arrays and QT-PCR showed that the expression of 133 genes was involved in signal transduction pathways, which included Toll-like receptor signaling, Wnt signaling, BMP signaling, insulin receptor signaling, TGF-beta signaling, GPCR signaling, small GTPase signaling, second-messenger-mediated signaling, MAPK signaling, JAK/STAT signaling, apoptosis and anti-apoptosis signaling and other signaling cascades. These signaling pathways may connect with each other to form a complex network to regulate not just immune responses but also other processes such as development and apoptosis. When transiently over-expressed in HEK293T cells, zfPGRP-SC inhibited NF-kappaB activity with and without lipopolysacharide (LPS) stimulation.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes