PUBLICATION

Repeated, noninvasive, high resolution spectral domain optical coherence tomography imaging of zebrafish embryos

Authors
Kagemann, L., Ishikawa, H., Zou, J., Charukamnoetkanok, P., Wollstein, G., Townsend, K.A., Gabriele, M.L., Bahary, N., Wei, X., Fujimoto, J.G., and Schuman, J.S.
ID
ZDB-PUB-081217-5
Date
2008
Source
Molecular Vision   14: 2157-2170 (Journal)
Registered Authors
Bahary, Nathan, Wei, Xiangyun
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Artifacts
  • Blood Vessels/anatomy & histology
  • Body Size
  • Embryo, Nonmammalian/anatomy & histology*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology*
  • Fertilization
  • Guanylate Cyclase/genetics
  • Heart/embryology
  • Imaging, Three-Dimensional*
  • Mutation/genetics
  • Tomography, Optical Coherence/methods*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
PubMed
19052656
Abstract
PURPOSE: To demonstrate a new imaging method for high resolution spectral domain optical coherence tomography (SD-OCT) for small animal developmental imaging. METHODS: Wildtype zebrafish that were 24, 48, 72, and 120 h post fertilization (hpf) and nok gene mutant (48 hpf) embryos were imaged in vivo. Three additional embryos were imaged twice, once at 72 hpf and again at 120 hpf. Images of the developing eye, brain, heart, whole body, proximal yolk sac, distal yolk sac, and tail were acquired. Three-dimensional OCT data sets (501 x 180 axial scans) were obtained as well as oversampled frames (8,100 axial scans) and repeated line scans (180 repeated frames). Scan volumes ranged from 750 x 750 microm to 3 x 3 mm, each 1.8 mm thick. Three-dimensional data sets allowed construction of C-mode slabs of the embryo. RESULTS: SD-OCT provided ultra-high resolution visualization of the eye, brain, heart, ear, and spine of the developing embryo as early as 24 hpf, and allowed development to be documented in each of these organ systems in consecutive sessions. Repeated line scanning with averaging optimized the visualization of static and dynamic structures contained in SD-OCT images. Structural defects caused by a mutation in the nok gene were readily observed as impeded ocular development, and enlarged pericardial cavities. CONCLUSIONS: SD-OCT allowed noninvasive, in vivo, ultra-high resolution, high-speed imaging of zebrafish embryos in their native state. The ability to measure structural and functional features repeatedly on the same specimen, without the need to sacrifice, promises to be a powerful tool in small animal developmental imaging.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes