PUBLICATION
Cytokinesis of neuroepithelial cells can divide their basal process before anaphase
- Authors
- Kosodo, Y., Toida, K., Dubreuil, V., Alexandre, P., Schenk, J., Kiyokage, E., Attardo, A., Mora-Bermúdez, F., Arii, T., Clarke, J.D., and Huttner, W.B.
- ID
- ZDB-PUB-081031-9
- Date
- 2008
- Source
- The EMBO journal 27(23): 3151-3163 (Journal)
- Registered Authors
- Alexandre, Paula, Clarke, Jon
- Keywords
- anillin, cell division, cell polarity, cleavage plane orientation, contractile ring
- MeSH Terms
-
- Actins/analysis
- Animals
- Cell Division*
- Cells, Cultured
- Chickens
- Contractile Proteins/analysis
- Cytokinesis*
- Cytoplasm/chemistry
- Genes, Reporter
- Green Fluorescent Proteins
- Mice
- Microscopy, Confocal
- Microscopy, Electron
- Microscopy, Video
- Neuroepithelial Cells/chemistry
- Neuroepithelial Cells/physiology*
- Recombinant Fusion Proteins/analysis
- Zebrafish
- PubMed
- 18971946 Full text @ EMBO J.
Citation
Kosodo, Y., Toida, K., Dubreuil, V., Alexandre, P., Schenk, J., Kiyokage, E., Attardo, A., Mora-Bermúdez, F., Arii, T., Clarke, J.D., and Huttner, W.B. (2008) Cytokinesis of neuroepithelial cells can divide their basal process before anaphase. The EMBO journal. 27(23):3151-3163.
Abstract
Neuroepithelial (NE) cells, the primary stem and progenitor cells of the vertebrate central nervous system, are highly polarized and elongated. They retain a basal process extending to the basal lamina, while undergoing mitosis at the apical side of the ventricular zone. By studying NE cells in the embryonic mouse, chick and zebrafish central nervous system using confocal microscopy, electron microscopy and time-lapse imaging, we show here that the basal process of these cells can split during M phase. Splitting occurred in the basal-to-apical direction and was followed by inheritance of the processes by either one or both daughter cells. A cluster of anillin, an essential component of the cytokinesis machinery, appeared at the distal end of the basal process in prophase and was found to colocalize with F-actin at bifurcation sites, in both proliferative and neurogenic NE cells. GFP-anillin in the basal process moved apically to the cell body prior to anaphase onset, followed by basal-to-apical ingression of the cleavage furrow in telophase. The splitting of the basal process of M-phase NE cells has implications for cleavage plane orientation and the relationship between mitosis and cytokinesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping