PUBLICATION

In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis

Authors
Caron, S.J., Prober, D., Choy, M., and Schier, A.F.
ID
ZDB-PUB-080902-27
Date
2008
Source
Development (Cambridge, England)   135(19): 3259-3269 (Journal)
Registered Authors
Caron, Sophie, Choy, Margaret, Prober, David, Schier, Alexander
Keywords
Neurogenesis, Trigeminal sensory ganglia, Trp, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental
  • Ion Channels/genetics
  • Ion Channels/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Models, Neurological
  • Neurogenesis*/genetics
  • Neurogenesis*/physiology
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Sensory Receptor Cells/cytology*
  • Sensory Receptor Cells/metabolism
  • Time Factors
  • Trigeminal Ganglion/cytology
  • Trigeminal Ganglion/embryology*
  • Trigeminal Ganglion/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
18755773 Full text @ Development
Abstract
Among sensory systems, the somatic sense is exceptional in its ability to detect a wide range of chemical, mechanical and thermal stimuli. How this sensory diversity is established during development remains largely elusive. We devised a method (BAPTISM) that uses the photoconvertible fluorescent protein Kaede to simultaneously analyze birthdate and cell fate in live zebrafish embryos. We found that trigeminal sensory ganglia are formed from early-born and late-born neurons. Early-born neurons give rise to multiple classes of sensory neurons that express different ion channels. By contrast, late-born neurons are restricted in their fate and do not form chemosensory neurons expressing the ion channel TrpA1b. Accordingly, larvae lacking early-born neurons do not respond to the TrpA1b agonist allyl isothiocyanate. These results indicate that the multimodal specification and function of trigeminal sensory ganglia depends on the timing of neurogenesis.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes