PUBLICATION
Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors
- Authors
- Delporte, F.M., Pasque, V., Devos, N., Manfroid, I., Voz, M.L., Motte, P., Biemar, F., Martial, J.A., and Peers, B.
- ID
- ZDB-PUB-080527-1
- Date
- 2008
- Source
- BMC Developmental Biology 8: 53 (Journal)
- Registered Authors
- Biemar, Frédéric, Delporte, Francois, Devos, Nathalie, Manfroid, Isabelle, Martial, Joseph A., Peers, Bernard, Voz, Marianne
- Keywords
- none
- MeSH Terms
-
- Pancreas/embryology
- Pancreas/metabolism*
- Sequence Homology, Nucleic Acid
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Paired Box Transcription Factors/genetics*
- Homeodomain Proteins/chemistry
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Eye Proteins/genetics*
- Transcription Factors/chemistry
- Transcription Factors/metabolism
- Enhancer Elements, Genetic*
- Retina/embryology
- Retina/metabolism
- Base Sequence
- Gene Expression Regulation, Developmental
- Trans-Activators/chemistry
- Trans-Activators/metabolism
- Promoter Regions, Genetic
- Conserved Sequence
- Multiprotein Complexes
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Animals
- Repressor Proteins/genetics*
- Animals, Genetically Modified
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/metabolism
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Molecular Sequence Data
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Telencephalon/embryology
- Telencephalon/metabolism
- PubMed
- 18485195 Full text @ BMC Dev. Biol.
Citation
Delporte, F.M., Pasque, V., Devos, N., Manfroid, I., Voz, M.L., Motte, P., Biemar, F., Martial, J.A., and Peers, B. (2008) Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors. BMC Developmental Biology. 8:53.
Abstract
BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells. Studies on the mouse Pax6 gene have shown that sequences upstream from the P0 promoter are required for expression in the lens and the pancreas; but there remain discrepancies regarding the precise location of the pancreatic regulatory elements. RESULTS: Due to genome duplication in the evolution of ray-finned fishes, zebrafish has two pax6 genes, pax6a and pax6b. While both zebrafish pax6 genes are expressed in the developing eye and nervous system, only pax6b is expressed in the endocrine cells of the pancreas. To investigate the cause of this differential expression, we used a combination of in silico, in vivo and in vitro approaches. We show that the pax6b P0 promoter targets expression to endocrine pancreatic cells and also to enteroendocrine cells, retinal neurons and the telencephalon of transgenic zebrafish. Deletion analyses indicate that strong pancreatic expression of the pax6b gene relies on the combined action of two conserved regulatory enhancers, called regions A and C. By means of gel shift assays, we detected binding of the homeoproteins PDX1, PBX and PREP to several cis-elements of these regions. In constrast, regions A and C of the zebrafish pax6a gene are not active in the pancreas, this difference being attributable to sequence divergences within two cis-elements binding the pancreatic homeoprotein PDX1. CONCLUSIONS: Our data indicate a conserved role of enhancers A and C in the pancreatic expression of pax6b and emphasize the importance of the homeoproteins PBX and PREP cooperating with PDX1, in activating pax6b expression in endocrine pancreatic cells. This study also provides a striking example of how adaptative evolution of gene regulatory sequences upon gene duplication progressively leads to subfunctionalization of the paralogous gene pair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping