|ZFIN ID: ZDB-PUB-080324-4|
Current perspectives in zebrafish reverse genetics: Moving forward
Skromne, I., and Prince, V.E.
|Source:||Developmental dynamics : an official publication of the American Association of Anatomists 237(4): 861-862 (Review)|
|Registered Authors:||Prince, Victoria E., Skromne, Isaac|
|Keywords:||reverse genetics, pharmacology, mRNA, over-expression, dominant negative, gain-of-function, loss-of-function, temporal regulation, caged RNA, heat shock, antisense, morpholino, peptide nucleic acid, ribozyme, RNA interference, siRNA, miRNA, dsRNA, transgenesis, retrovirus, transposons, Tol2, Cre/lox, Tet-on, Gal4/UAS, insertional mutagenesis, target gene inactivation, homologous recombination, target-selected mutagenesis, TILLING, retinoic acid|
|PubMed:||18330930 Full text @ Dev. Dyn.|
Skromne, I., and Prince, V.E. (2008) Current perspectives in zebrafish reverse genetics: Moving forward. Developmental dynamics : an official publication of the American Association of Anatomists. 237(4):861-862.
ABSTRACTse of the zebrafish as a model of vertebrate development and disease has expanded dramatically over the past decade. While many articles have discussed the strengths of zebrafish forward genetics (the phenotype-driven approach), there has been less emphasis on equally important and frequently used reverse genetics (the candidate gene-driven approach). Here we review both current and prospective reverse genetic techniques that are applicable to the zebrafish model. We include discussion of pharmacological approaches, popular gain-of-function and knockdown approaches, and gene targeting strategies. We consider the need for temporal and spatial control over gain/loss of gene function, and discuss available and developing techniques to achieve this end. Our goal is both to reveal the current technical advantages of the zebrafish and to highlight those areas where work is still required to allow this system to be exploited to full advantage.
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