ZFIN ID: ZDB-PERS-010525-1
Skromne, Isaac
Email: iskromne@richmond.edu
URL: http://www.skromnelab.com/
Affiliation: Skromne Lab
Address: Department of Biology University of Richmond 138 UR Drive - B322 Richmond, VA 23173
Country: United States
Phone: (804) 289-8235
Fax: (804) 289-8871
ORCID ID: 0000-0003-2369-7210

* Assistant Professor, 2017-present, Biology Department, The University of Richmond
* Assistant Professor, 2008-2017, Biology Department, The University of Miami
* Research Professional, 2005-2007, The University of Chicago, Chicago, IL, USA
* Postdoctoral Scholar, 2003-2005, The University of Chicago, Chicago, IL, USA
* Postdoctoral Fellow, 2001-2003, The University of Chicago, Chicago, IL, USA
* Visiting Research Fellow, 2000-2001, Princeton University, Princeton, NJ, USA
* Embryology Fellow, 2000, Marine Biological Laboratory, Woods Hole, MA, USA
* Ph.D. with Honors, 2000, Columbia University, New York, NY, USA
* M. Phil., 1996, Columbia University, New York, NY, USA
* M.S., 1995, Columbia University, New York, NY, USA
* B.S. with Distinction, 1994, National Autonomous University of Mexico, Mexico City, Mexico

My group is interested in how two fundamental developmental processes, segmentation and patterning, are regulated and coordinated during vertebrate embryogenesis to generate morphological complexity. While segmentation subdivides the embryonic body plan into an array of morphologically similar units or segments, patterning processes assign different positional identities to such units based on their anterior-posterior position along the main body axis of the embryo. What are the mechanisms that coordinate segmentation and patterning within and between different tissues such as the nervous system and mesoderm (muscle and bone forming tissue)? What are the conserved and divergent aspects of this machinery that drive variation in segment number and/or identity between vertebrates?

We are addressing these questions using the chicken and zebrafish embryos as model organisms. This allows us to combine classic embryological techniques with powerful molecular and genetic approaches. By using two evolutionarily distant organisms, we expect not only to elucidate the core molecular processes underlying segmentation and patterning of the nervous system and mesoderm, but also the source of diversity in vertebrate segment number and form. Furthermore, we expect this research to have important medical implications as it directly addresses developmental processes underlying common birth defects such as open neural tube or congenital vertebral malsegmentation.

Peng, Z., Miyanji, E.H., Zhou, Y., Pardo, J., Hettiarachchi, S.D., Li, S., Blackwelder, P.L., Skromne, I., Leblanc, R.M. (2017) Carbon dots: promising biomaterials for bone-specific imaging and drug delivery. Nanoscale. 9:17533-17543
Chang, J., Skromne, I., Ho, R.K. (2016) CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition. Developmental Biology. 410(2):178-89
Hayward, A.G., Joshi, P., Skromne, I. (2015) Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4. Developmental dynamics : an official publication of the American Association of Anatomists. 244(12):1564-73
Lee, K., Skromne, I. (2014) Retinoic acid regulates size, pattern and alignment of tissues at the head-trunk transition. Development (Cambridge, England). 141:4375-84
Yariz, K.O., Duman, D., Seco, C.Z., Dallman, J., Huang, M., Peters, T.A., Sirmaci, A., Lu, N., Schraders, M., Skromne, I., Oostrik, J., Diaz-Horta, O., Young, J.I., Tokgoz-Yilmaz, S., Konukseven, O., Shahin, H., Hetterschijt, L., Kanaan, M., Oonk, A.M., Edwards, Y.J., Li, H., Atalay, S., Blanton, S., Desmidt, A.A., Liu, X.Z., Pennings, R.J., Lu, Z., Chen, Z.Y., Kremer, H., and Tekin, M. (2012) Mutations in OTOGL, Encoding the Inner Ear Protein Otogelin-like, Cause Moderate Sensorineural Hearing Loss. American journal of human genetics. 91(5):872-882
Skromne, I., and Prince, V.E. (2008) Current perspectives in zebrafish reverse genetics: Moving forward. Developmental dynamics : an official publication of the American Association of Anatomists. 237(4):861-862
Skromne, I., Thorsen, D., Hale, M., Prince, V.E., and Ho, R.K. (2007) Repression of the hindbrain developmental program by Cdx factors is required for the specification of the vertebrate spinal cord. Development (Cambridge, England). 134(11):2147-2158
Skromne, I., Kikuchi, Y., Stainier, D., and Ho, R.K. (2002) The para-hox gene caudal is required for tail formation in zebrafish. Developmental Biology. 247(2):472-473

Joshi, P. and Skromne, I. (2020) A theoretical model of neural maturation in the developing chick spinal cord. PLOS One. 15:e0244219

Joshi, P., Darr, A. J., and Skromne, I. (2019) CDX4 regulates the progression of neural maturation in the spinal cord. Development Biology 449:132-142.

Bertocchini, F., Skromne, I. and Stern, C. D. (2004) Determination of embryonic polarity in a regulative system: evidence for endogenous inhibitors acting sequentially during primitive streak formation in the chick embryo. Development 131, 3381-3390.

Skromne, I. and Stern, C. D. (2002) A hierarchy of gene expression accompanying induction of the primitive streak by Vg1 in the chick embryo. Mech Dev. 114, 115-118.

Skromne, I. and Stern, C. D. (2001) Interactions between Wnt and Vg1 signalling pathways initiate primitive streak formation in the chick embryo. Development 128, 2915-2927.

Foley, A. C., Skromne, I. and Stern, C. D. (2000) Reconciling different models of forebrain induction and patterning: a dual role for the hypoblast. Development 127, 3839-3854.

Bachvarova, R. F., Skromne, I. and Stern, C. D. (1998) Induction of primitive streak and Hensen’s node by the posterior marginal zone in the early chick embryo. Development 125, 3521-3534.

Shah, S. B., Skromne, I., Humme, C. R., Kessler, D. S., Lee, K. J., Stern, C. D. and Dodd, J. (1997) Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. Development 124, 5127-5138.