PUBLICATION

SIX2 and BMP4 Mutations Associate With Anomalous Kidney Development

Authors
Weber, S., Taylor, J.C., Winyard, P., Baker, K.F., Sullivan-Brown, J., Schild, R., Knüppel, T., Zurowska, A.M., Caldas-Alfonso, A., Litwin, M., Emre, S., Ghiggeri, G.M., Bakkaloglu, A., Mehls, O., Antignac, C., Schaefer, F., and Burdine, R.D.
ID
ZDB-PUB-080306-31
Date
2008
Source
Journal of the American Society of Nephrology : JASN   19(5): 891-903 (Journal)
Registered Authors
Baker, Kari, Burdine, Rebecca, Sullivan-Brown, Jessica
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/genetics*
  • DNA Mutational Analysis
  • Disease Models, Animal
  • Gene Expression Regulation, Developmental
  • Genotype
  • Homeodomain Proteins/genetics*
  • Humans
  • Kidney/abnormalities*
  • Kidney/physiology*
  • Molecular Sequence Data
  • Nephrons/abnormalities
  • Nephrons/physiology
  • Nerve Tissue Proteins/genetics*
  • PAX2 Transcription Factor/genetics
  • Phenotype
  • Renal Insufficiency/genetics*
  • Renal Insufficiency/pathology*
  • Renal Insufficiency/physiopathology
  • WT1 Proteins/genetics
  • Zebrafish
  • Zebrafish Proteins/genetics
PubMed
18305125 Full text @ J. Am. Soc. Nephrol.
Abstract
Renal hypodysplasia (RHD) is characterized by reduced kidney size and/or maldevelopment of the renal tissue following abnormal organogenesis. Mutations in renal developmental genes have been identified in a subset of affected individuals. Here, we report the first mutations in BMP4 and SIX2 identified in patients with RHD. We detected 3 BMP4 mutations in 5 RHD patients, and 3 SIX2 mutations in 5 different RHD patients. Overexpression assays in zebrafish demonstrated that these mutations affect the function of Bmp4 and Six2 in vivo. Overexpression of zebrafish six2.1 and bmp4 resulted in dorsalization and ventralization, respectively, suggesting opposing roles in mesendoderm formation. When mutant constructs containing the identified human mutations were overexpressed instead, these effects were attenuated. Morpholino knockdown of bmp4 and six2.1 affected glomerulogenesis, suggesting specific roles for these genes in the formation of the pronephros. In summary, these studies implicate conserved roles for Six2 and Bmp4 in the development of the renal system. Defects in these proteins could affect kidney development at multiple stages, leading to the congenital anomalies observed in patients with RHD.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping