ZFIN ID: ZDB-PUB-080227-7
Identification of ZBP-89 as a Novel GATA-1 Associated Transcription Factor Involved in Megakaryocytic and Erythroid Development
Woo, A.J., Moran, T.B., Schindler, Y., Choe, S.K., Langer, N.B., Sullivan, M.R., Fujiwara, Y., Paw, B.H., and Cantor, A.B.
Date: 2008
Source: Molecular and cellular biology   28(8): 2675-2689 (Journal)
Registered Authors: Choe, Seong-Kyu, Paw, Barry
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation*
  • Cell Line
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/isolation & purification
  • DNA-Binding Proteins/metabolism*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Erythroid Cells/cytology
  • Erythroid Cells/metabolism*
  • GATA1 Transcription Factor/chemistry
  • GATA1 Transcription Factor/genetics
  • GATA1 Transcription Factor/isolation & purification
  • GATA1 Transcription Factor/metabolism*
  • Megakaryocytes/cytology
  • Megakaryocytes/metabolism*
  • Mice
  • Molecular Sequence Data
  • Ploidies
  • Protein Binding
  • Rats
  • Transcription Factors/genetics
  • Transcription Factors/isolation & purification
  • Transcription Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 18250154 Full text @ Mol. Cell. Biol.
A complete understanding of the transcriptional regulation of developmental lineages requires that all relevant factors be identified. Here, we have taken a proteomic approach to identify novel proteins associated with GATA-1, a lineage-restricted zinc finger transcription factor required for terminal erythroid and megakaryocytic maturation. We identify the Kr├╝ppel-type zinc finger transcription factor ZBP-89 as a component of multiprotein complexes involving GATA-1 and its essential cofactor Friend of GATA-1 (FOG-1). Using chromatin immunoprecipitation assays, we show that GATA-1 and ZBP-89 co-occupy cis-regulatory elements of certain erythroid and megakaryocyte-specific genes, including an enhancer of the GATA-1 gene itself. Loss-of-function studies in zebrafish and mice demonstrate an in vivo requirement for ZBP-89 in megakaryopoiesis and definitive erythropoiesis, but not primitive erythropoiesis, phenocopying aspects of FOG-1 and GATA-1 deficient animals. These findings identify ZBP-89 as a novel transcription factor involved in erythroid and megakaryocytic development and suggest that it serves a cooperative function with GATA-1 and/or FOG-1 in a developmental stage-specific manner.