|ZFIN ID: ZDB-PUB-080227-7|
Identification of ZBP-89 as a Novel GATA-1 Associated Transcription Factor Involved in Megakaryocytic and Erythroid Development
Woo, A.J., Moran, T.B., Schindler, Y., Choe, S.K., Langer, N.B., Sullivan, M.R., Fujiwara, Y., Paw, B.H., and Cantor, A.B.
|Source:||Molecular and cellular biology 28(8): 2675-2689 (Journal)|
|Registered Authors:||Choe, Seong-Kyu, Paw, Barry|
|PubMed:||18250154 Full text @ Mol. Cell. Biol.|
Woo, A.J., Moran, T.B., Schindler, Y., Choe, S.K., Langer, N.B., Sullivan, M.R., Fujiwara, Y., Paw, B.H., and Cantor, A.B. (2008) Identification of ZBP-89 as a Novel GATA-1 Associated Transcription Factor Involved in Megakaryocytic and Erythroid Development. Molecular and cellular biology. 28(8):2675-2689.
ABSTRACTA complete understanding of the transcriptional regulation of developmental lineages requires that all relevant factors be identified. Here, we have taken a proteomic approach to identify novel proteins associated with GATA-1, a lineage-restricted zinc finger transcription factor required for terminal erythroid and megakaryocytic maturation. We identify the Krüppel-type zinc finger transcription factor ZBP-89 as a component of multiprotein complexes involving GATA-1 and its essential cofactor Friend of GATA-1 (FOG-1). Using chromatin immunoprecipitation assays, we show that GATA-1 and ZBP-89 co-occupy cis-regulatory elements of certain erythroid and megakaryocyte-specific genes, including an enhancer of the GATA-1 gene itself. Loss-of-function studies in zebrafish and mice demonstrate an in vivo requirement for ZBP-89 in megakaryopoiesis and definitive erythropoiesis, but not primitive erythropoiesis, phenocopying aspects of FOG-1 and GATA-1 deficient animals. These findings identify ZBP-89 as a novel transcription factor involved in erythroid and megakaryocytic development and suggest that it serves a cooperative function with GATA-1 and/or FOG-1 in a developmental stage-specific manner.