PUBLICATION

Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development

Authors

Manfroid, I., Delporte, F., Baudhuin, A., Motte, P., Neumann, C.J., Voz, M.L., Martial, J.A., and Peers, B.

ID

ZDB-PUB-071023-19

Date

2007

Source

Development (Cambridge, England) 134(22): 4011-4021 (Journal)

Registered Authors

Baudhuin, Ariane, Delporte, Francois, Manfroid, Isabelle, Martial, Joseph A., Neumann, Carl J., Peers, Bernard, Voz, Marianne

Keywords

none

MeSH Terms

Cell Differentiation
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*
Animals, Genetically Modified
Organ Specificity
Zebrafish/embryology
LIM-Homeodomain Proteins
Models, Biological
Signal Transduction/genetics
Embryo, Nonmammalian
Endoderm/metabolism
Endoderm/physiology*
Animals
Cell Communication/physiology
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism
Transcription Factors
Fibroblast Growth Factor 10
Pancreas/embryology*
Pancreas/metabolism
Fibroblast Growth Factors/genetics
Fibroblast Growth Factors/physiology*
Mesoderm/metabolism
Mesoderm/physiology*
Gene Expression Regulation, Developmental

(all 26)

PubMed

17942484 Full text @ Development

Abstract

In amniotes, the pancreatic mesenchyme plays a crucial role in pancreatic epithelium growth, notably through the secretion of fibroblast growth factors. However, the factors involved in the formation of the pancreatic mesenchyme are still largely unknown. In this study, we characterize, in zebrafish embryos, the pancreatic lateral plate mesoderm, which is located adjacent to the ventral pancreatic bud and is essential for its specification and growth. We firstly show that the endoderm, by expressing the fgf24 gene at early stages, triggers the patterning of the pancreatic lateral plate mesoderm. Based on the expression of isl1, fgf10 and meis genes, this tissue is analogous to the murine pancreatic mesenchyme. Secondly, Fgf10 acts redundantly with Fgf24 in the pancreatic lateral plate mesoderm and they are both required to specify the ventral pancreas. Our results unveil sequential signaling between the endoderm and mesoderm that is critical for the specification and growth of the ventral pancreas, and explain why the zebrafish ventral pancreatic bud generates the whole exocrine tissue.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
cp	AB	control	Protruding-mouth		liver	ISH	Fig. 5
cp	AB + MO1-fgf24	standard conditions	Protruding-mouth		liver	ISH	Fig. 5
etv4	AB	standard conditions	Prim-5		posterior lateral plate mesoderm	ISH	Fig. 6
etv4	AB	standard conditions	Prim-15		posterior lateral plate mesoderm	ISH	Fig. 6
etv4	AB	control	Prim-25		posterior lateral plate mesoderm	ISH	Fig. 6
etv4	AB + MO1-fgf24	standard conditions	Prim-25	Not Detected	posterior lateral plate mesoderm	ISH	Fig. 6
etv5b	AB	standard conditions	Prim-5		posterior lateral plate mesoderm	ISH	Fig. S3
etv5b	AB	standard conditions	Prim-15		posterior lateral plate mesoderm	ISH	Fig. 6
etv5b	AB	standard conditions	Prim-5		posterior lateral plate mesoderm	ISH	Fig. 6
fgf10a	AB	standard conditions	Prim-25		pectoral fin	ISH	Fig. 1

1 - 10 of 93

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-fgf10a	standard conditions	Protruding-mouth	fin bud aplastic, abnormal	Fig. 7
AB + MO1-fgf10a + MO1-fgf24	standard conditions	Protruding-mouth	fin bud aplastic, abnormal	Fig. 7
AB + MO1-fgf24	standard conditions	Protruding-mouth	fin bud aplastic, abnormal	Fig. 5

1 - 3 of 3

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
sox32_unspecified	Unspecified	sox32
t22030	Point Mutation	fgf24
tbvbo	Point Mutation	fgf10a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
fgf10a	MO1-fgf10a	MRPHLNO
fgf24	MO1-fgf24	MRPHLNO
isl1a	MO5-isl1a	MRPHLNO
meis3	MO3-meis3	MRPHLNO

Fish

Fish
AB
AB + MO1-fgf10a
AB + MO1-fgf10a + MO1-fgf24
AB + MO1-fgf24
AB + MO3-meis3
AB + MO5-isl1a
fgf10a^tbvbo/tbvbo
fgf24^{t22030/t22030}
sox32^unspecified

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Manfroid et al., 2007 ZDB-PUB-071023-19 PMID:17942484

Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development

Manfroid et al., 2007

ZDB-PUB-071023-19
PMID:17942484