ZFIN ID: ZDB-PUB-070912-3
T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine
Muncan, V., Faro, A., Haramis, A.P., Hurlstone, A.F., Wienholds, E., van Es, J., Korving, J., Begthel, H., Zivkovic, D., and Clevers, H.
Date: 2007
Source: EMBO reports   8(10): 966-973 (Journal)
Registered Authors: Clevers, Hans, Haramis, Anna-Pavlina, Wienholds, Erno
Keywords: none
MeSH Terms:
  • Animals
  • Cell Proliferation*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Genotype
  • Immunohistochemistry
  • In Situ Hybridization
  • Intestines/cytology
  • Intestines/metabolism*
  • Male
  • Mutation
  • Phenotype
  • Time Factors
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology
PubMed: 17823612 Full text @ EMBO Rep.
Previous studies have shown that Wnt signals, relayed through beta-catenin and T-cell factor 4 (Tcf4), are essential for the induction and maintenance of crypts in mice. We have now generated a tcf4 (tcf7l2) mutant zebrafish by reverse genetics. We first observe a phenotypic defect at 4 weeks post-fertilization (wpf), leading to death at about 6 wpf. The phenotype comprises a loss of proliferation at the base of the intestinal folds of the middle and distal parts of the intestine. The proximal intestine represents an independent compartment, as it expresses sox2 in the epithelium and barx1 in the surrounding mesenchyme, which are early stomach markers in higher vertebrates. Zebrafish are functionally stomach-less, but the proximal intestine might share its ontogeny with the mammalian stomach. Rare adult homozygous tcf4(-/-) 'escapers' show proliferation defects in the gut epithelium, but have no other obvious abnormalities. This study underscores the involvement of Tcf4 in maintaining proliferative self-renewal in the intestine throughout life.