ZFIN ID: ZDB-PERS-980618-11
Haramis, Anna-Pavlina
Email: a.haramis@biology.leidenuniv.nl
URL: http://www.science.leidenuniv.nl/index.php/ibl/haramis
Affiliation: Haramis Lab
Address: Institute of Biology Leiden (IBL), Leiden University Sylvius Laboratory, Room 06.05.16b Sylviusweg 72, 2333 BE, Leiden The Netherlands
Country: Netherlands
Phone: +31 71 527 4782

We are using the zebrafish as an experimental model system to elucidate the developmental roles of tumor suppressors and oncogenes and to dissect molecular mechanisms that when subverted contribute to cancer.We perform gain and loss of function experiments in embryos and characterize the phenotype of zebrafish mutants in these genes.
We are particularly interested in
• Analysis of cell polarity and energy metabolism during normal development and cancer
• Analysis of the developmental roles of the Polycomb group proteins
• Study of deubiquitination enzymes acting on the Wnt signaling pathway in zebrafish

van Veen, M., Mans, L.A., Matas-Rico, E., van Pelt, J., Perrakis, A., Moolenaar, W.H., Haramis, A.G. (2017) Glycerophosphodiesterase GDE2/GDPD5 affects pancreas differentiation in zebrafish. The international journal of biochemistry & cell biology. 94:71-78
Mans, L.A., Querol Cano, L., van Pelt, J., Giardoglou, P., Keune, W.J., Haramis, A.G. (2017) The tumor suppressor LKB1 regulates starvation-induced autophagy under systemic metabolic stress. Scientific Reports. 7:7327
Stijf-Bultsma, Y., Sommer, L., Tauber, M., Baalbaki, M., Giardoglou, P., Jones, D.R., Gelato, K.A., van Pelt, J., Shah, Z., Rahnamoun, H., Toma, C., Anderson, K.E., Hawkins, P., Lauberth, S.M., Haramis, A.P., Hart, D., Fischle, W., Divecha, N. (2015) The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output. Molecular Cell. 58:453-67
van der Velden, Y.U., Wang, L., Querol Cano, L., and Haramis, A.P. (2013) The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One. 8(9):e73997
Elouarrat, D., van der Velden, Y., Jones, D.R., Moolenaar, W.H., Divecha, N., and Haramis, A.P. (2013) Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development. The international journal of biochemistry & cell biology. 45(7):1293-301
van der Velden, Y.U., Wang, L., van Lohuizen, M., and Haramis, A.P. (2012) The Polycomb group protein Ring1b is essential for pectoral fin development. Development (Cambridge, England). 139(12):2210-2220
van der Velden, Y.U., and Haramis, A.P. (2011) Insights from model organisms on the functions of the tumor suppressor protein LKB1: zebrafish chips in. Aging. 3(4):363-367
van der Velden, Y.U., Wang, L., Zevenhoven, J., van Rooijen, E., van Lohuizen, M., Giles, R.H., Clevers, H., and Haramis, A.P. (2011) The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 108(11):4358-4363
Brennan, C., Dosch, R., Haramis, A.P., Luckenbacher, T., Martinez-Morales, J.R., Moro, E., Polok, B., Ramesh, T.M., Russell, C., Argenton, F., and Strähle, U. (2010) Report of the European Zebrafish Principal Investigator Meeting in Padua, Italy, March 18-22, 2010. Zebrafish. 7(3):305-310
Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., and Zon, L.I. (2008) APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development. Developmental Biology. 320(1):161-174
Hawkins, T.A., Haramis, A.P., Etard, C., Prodromou, C., Vaughan, C.K., Ashworth, R., Ray, S., Behra, M., Holder, N., Talbot, W.S., Pearl, L.H., Strähle, U., and Wilson, S.W. (2008) The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis. Development (Cambridge, England). 135(6):1147-1156
Muncan, V., Faro, A., Haramis, A.P., Hurlstone, A.F., Wienholds, E., van Es, J., Korving, J., Begthel, H., Zivkovic, D., and Clevers, H. (2007) T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine. EMBO reports. 8(10):966-973
Haramis, A.P., Hurlstone, A., van der Velden, Y., Begthel, H., van den Born, M., Offerhaus, G.J., and Clevers, H.C. (2006) Adenomatous polyposis coli-deficient zebrafish are susceptible to digestive tract neoplasia. EMBO reports. 7(4):444-449
Hurlstone, A.F., Haramis, A.P., Wienholds, E., Begthel, H., Korving, J., van Eeden, F., Cuppen, E., Zivkovic, D., Plasterk, R.H., and Clevers, H. (2003) The Wnt/beta-catenin pathway regulates cardiac valve formation. Nature. 425(6958):633-637

Haramis APG, Begthel H, van de Born M, van Es J, Jonkers S, Offerhaus GJA, and Clevers H (2004). De novo crypt formation and Juvenile Polyposis on BMP inhibition in mouse intestine. Science: 303, 1684-1686.
Zuniga A, Michos O, Spitz F, Haramis APG, Panman L, Galli A, Vintersten K, Klasen C, Mansfield W, Kuc S, Duboule D, Dono R, Zeller R (2004). Mouse limb deformity mutations disrupt a global control region within the large regulatory landscape required for Gremlin expression. Genes Dev. 18(13), 1553-1564.
van de Wetering M, Sancho E, Verweij C, de Lau W, Oving I, Hurlstone A, van der Horn K, Batlle E, Coudreuse D, Haramis APG, Tjon-Pon-Fong M, Moerer P, van den Born M, Soete G, Pals S, Eilers M, Medema R, and Hans Clevers (2002). The ²-Catenin/TCF-4 Complex Imposes a Crypt Progenitor Phenotype on Colorectal Cancer Cells. Cell: 111, 241-250.
Haramis APG*, Zuniga A*, McMahon AP, and Zeller R (1999). Signal relay by BMP antagonism controls the SHH/FGF4 feedback loop in vertebrate limb buds. Nature: 401, 598-602.
Zeller R, Haramis AG, Zuniga A, Mc. Guigan C, Dono R, Davidson G, Chabanis S, and Gibson T (1999). Formin defines a novel family of morpho-regulatory genes and functions in establishment of the polarising region. Cell and Tissue Research: 296 (1), 85-93.
Haramis AG, Brown JM and Zeller R (1995). The limb deformity mutation disrupts the SHH/FGF-4 feedback loop and regulation of 5' Hox-D genes during limb pattern formation. Development: 121, 4237-4245.