The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis
- Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U.
- Developmental Biology 308(1): 133-143 (Journal)
- Registered Authors
- Behra, Martine, Etard, Christelle, Fischer, Nadine, Geisler, Robert, Hutcheson, David, Strähle, Uwe
- Zebrafish, Chaperone, Co-chaperone, Muscle damage, hsp90a, UCS factor
- MeSH Terms
- Amino Acid Sequence
- Animals, Genetically Modified
- Base Sequence
- Gene Expression Regulation, Developmental
- HSP90 Heat-Shock Proteins/genetics
- HSP90 Heat-Shock Proteins/metabolism*
- In Vitro Techniques
- Microscopy, Electron, Transmission
- Molecular Sequence Data
- Muscle Development/genetics
- Muscle Development/physiology*
- Muscle Proteins/genetics
- Muscle Proteins/metabolism*
- Muscle, Skeletal/embryology
- Muscle, Skeletal/metabolism
- Muscle, Skeletal/ultrastructure
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 17586488 Full text @ Dev. Biol.
Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U. (2007) The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis. Developmental Biology. 308(1):133-143.
Contraction of muscles is mediated by highly organized arrays of myosin motor proteins. We report here the characterization of a mutation of a UCS gene named steif/unc-45b that is required for the formation of ordered myofibrils in both the skeletal and cardiac muscles of zebrafish. We show that Steif/Unc-45b interacts with the chaperone Hsp90a in vitro. The two genes are co-expressed in the skeletal musculature and knockdown of Hsp90a leads to impaired myofibril formation in the same manner as lack of Steif/Unc-45b activity. Transcripts of both genes are up-regulated in steif mutants suggesting co-regulation of the two genes. Our data indicate a requirement of Steif/unc-45b and Hsp90a for the assembly of the contractile apparatus in the vertebrate skeletal musculature.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes