ZFIN ID: ZDB-PUB-070625-12
RAD marker microarrays enable rapid mapping of zebrafish mutations
Miller, M.R., Atwood, T.S., Eames, B.F., Eberhart, J.K., Yan, Y.L., Postlethwait, J.H., and Johnson, E.A.
Date: 2007
Source: Genome biology   8(6): R105 (Journal)
Registered Authors: Eberhart, Johann, Postlethwait, John H., Yan, Yi-Lin
Keywords: none
MeSH Terms:
  • Animals
  • Chromosome Mapping/economics
  • Chromosome Mapping/methods*
  • Female
  • Male
  • Mutation
  • Oligonucleotide Array Sequence Analysis/methods
  • Polymerase Chain Reaction/methods
  • Restriction Mapping
  • Zebrafish/genetics*
PubMed: 17553171 Full text @ Genome Biol.
We constructed a RAD marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and an attractive option for mapping in other organisms.