PUBLICATION

Bmp and Fgf signaling are essential for liver specification in zebrafish

Authors
Shin, D., Shin, C.H., Tucker, J., Ober, E.A., Rentzsch, F., Poss, K.D., Hammerschmidt, M., Mullins, M.C., and Stainier, D.Y.
ID
ZDB-PUB-070523-16
Date
2007
Source
Development (Cambridge, England)   134(11): 2041-2050 (Journal)
Registered Authors
Hammerschmidt, Matthias, Mullins, Mary C., Ober, Elke, Poss, Kenneth D., Rentzsch, Fabian, Shin, Chong, Shin, Donghun, Stainier, Didier
Keywords
hhex, prox1, alk8 (acvr1), Competence, Endoderm, Hepatocyte, Zebrafish
MeSH Terms
  • GATA Transcription Factors/metabolism
  • Repressor Proteins/metabolism
  • Tumor Suppressor Proteins/metabolism
  • Endoderm/cytology
  • Endoderm/physiology
  • Hot Temperature
  • Liver/embryology*
  • Liver/metabolism
  • Bone Morphogenetic Protein Receptors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/metabolism
  • In Situ Hybridization
  • Signal Transduction/genetics
  • Signal Transduction/physiology*
  • Activin Receptors, Type I/metabolism
  • Receptors, Fibroblast Growth Factor/metabolism*
  • Cell Differentiation/physiology
  • Morphogenesis/physiology*
  • Animals
  • DNA Primers/genetics
  • Homeodomain Proteins/metabolism
  • Gene Expression Profiling
(all 23)
PubMed
17507405 Full text @ Development
Abstract
Based on data from in vitro tissue explant and ex vivo cell/bead implantation experiments, Bmp and Fgf signaling have been proposed to regulate hepatic specification. However, genetic evidence for this hypothesis has been lacking. Here, we provide in vivo genetic evidence that Bmp and Fgf signaling are essential for hepatic specification. We utilized transgenic zebrafish that overexpress dominant-negative forms of Bmp or Fgf receptors following heat-shock induction. These transgenes allow one to bypass the early embryonic requirements for Bmp and Fgf signaling, and also to completely block Bmp or Fgf signaling. We found that the expression of hhex and prox1, the earliest liver markers in zebrafish, was severely reduced in the liver region when Bmp or Fgf signaling was blocked just before hepatic specification. However, hhex and prox1 expression in adjacent endodermal and mesodermal tissues appeared unaffected by these manipulations. Additional genetic studies indicate that the endoderm maintains competence for Bmp-mediated hepatogenesis over an extended window of embryonic development. Altogether, these data provide the first genetic evidence that Bmp and Fgf signaling are essential for hepatic specification, and suggest that endodermal cells remain competent to differentiate into hepatocytes for longer than anticipated.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
gata4MO1-gata4MRPHLNO
gata6MO1-gata6MRPHLNO
1 - 2 of 2
Show
Fish
Fish
acvr1ltm110b/+; p0Tg
acvr1ltm110b/tm110b (TU)
fr13Tg/+
fr13Tg/+; pd1Tg/+
pd1Tg/+
pd1Tg/+
w30Tg/+ (AB)
WT
WT + MO1-gata4
WT + MO1-gata4 + MO1-gata6
1 - 10 of 11
Show
Antibodies
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
GFPEFGGFP
1 - 2 of 2
Show
Mapping
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Citation
Shin, D., Shin, C.H., Tucker, J., Ober, E.A., Rentzsch, F., Poss, K.D., Hammerschmidt, M., Mullins, M.C., and Stainier, D.Y. (2007) Bmp and Fgf signaling are essential for liver specification in zebrafish. Development (Cambridge, England). 134(11):2041-2050.