PUBLICATION

PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish

Authors
Feldner, J., Reimer, M.M., Schweitzer, J., Wendik, B., Meyer, D., Becker, T., and Becker, C.G.
ID
ZDB-PUB-070513-8
Date
2007
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   27(18): 4978-4983 (Journal)
Registered Authors
Becker, Catherina G., Becker, Thomas, Feldner, Julia, Meyer, Dirk, Reimer, Michell M., Schweitzer, Jörn, Wendik, Bjoern
Keywords
primary motor neurons, pioneer axons, neuropilin, semaphorin, zebrafish, development
MeSH Terms
  • Animals
  • Axons/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/physiology*
  • Motor Neurons/metabolism*
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/physiology*
  • Spinal Cord/embryology*
  • Spinal Cord/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
17475806 Full text @ J. Neurosci.
Abstract
The pioneering primary motor axons in the zebrafish trunk are guided by multiple cues along their pathways. Plexins are receptor components for semaphorins that influence motor axon growth and path finding. We cloned plexinA3 in zebrafish and localized plexinA3 mRNA in primary motor neurons during axon outgrowth. Antisense morpholino knock-down led to substantial errors in motor axon growth. Errors comprised aberrant branching of primary motor nerves as well as additional exit points of axons from the spinal cord. Excessively branched and supernumerary nerves were found in both ventral and dorsal pathways of motor axons. The trunk environment and several other types of axons, including trigeminal axons, were not detectably affected by plexinA3 knock-down. RNA overexpression rescued all morpholino effects. Synergistic effects of combined morpholino injections indicate interactions of plexinA3 with semaphorin3A homologs. Thus, plexinA3 is a crucial receptor for axon guidance cues in primary motor neurons.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes