PUBLICATION

Spatiotemporal expression of smooth muscle markers in developing zebrafish gut

Authors

Georgijevic, S., Subramanian, Y., Rollins, E.L., Starovic-Subota, O., Tang, A.C., Childs, S.J.

ID

ZDB-PUB-070513-6

Date

2007

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 236(6): 1623-1632 (Journal)

Registered Authors

Childs, Sarah J., Georgijevic, Sonja, Rollins, Evvi-Lynn

Keywords

smooth muscle, α-smooth muscle actin, SM22α, CPI-17, nonmuscle myosin heavy chain, smoothelin, tropomyosin, gut, swim bladder

MeSH Terms

Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Air Sacs/embryology
Air Sacs/metabolism
Digestive System/embryology*
Digestive System/metabolism*
Conserved Sequence
Phylogeny
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Gene Expression Regulation, Developmental*
Molecular Sequence Data
Zebrafish Proteins/chemistry
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Humans
Biomarkers
Animals
Sequence Alignment
Aging/physiology
Time Factors
Amino Acid Sequence
Muscle, Smooth/metabolism*

(all 24)

PubMed

17474123 Full text @ Dev. Dyn.

Abstract

Smooth muscle is important for the contractility and elasticity of visceral organs. The zebrafish is an excellent model for understanding embryonic development, yet due to a lack of appropriate markers, visceral smooth muscle development remains poorly characterized. Here, we develop markers and trace the development of gut and swim bladder smooth muscle in embryonic and juvenile fish. The first smooth muscle marker we detect in the vicinity of the gut is the myoblast marker nonmuscle myosin heavy chain-b at 50 hours postfertilization (hpf), followed by the early smooth muscle markers SM22alpha-b, and alpha-smooth muscle actin at 56 and 60 hpf, respectively. Markers of more differentiated smooth muscle, smoothelin-b and cpi-17, appear by 3 days postfertilization (dpf). Tropomyosin, a relatively late marker, is first expressed at 4 dpf. We find that smooth muscle marker expression in the swim bladder follows the same sequence of marker expression as the gut, but markers have a temporal delay reflecting the later formation of swim bladder smooth muscle.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
acta2	WT	standard conditions	Protruding-mouth	intestine	ISH	Fig. 3
acta2	WT	standard conditions	Day 4	intestine	ISH	Fig. 3
acta2	WT	standard conditions	Pec-fin	intestine	ISH	Fig. 3
acta2	WT	standard conditions	Day 5	intestine	ISH	Fig. 3
acta2	WT	standard conditions	Day 5	swim bladder	ISH	Fig. 3
acta2	WT	standard conditions	Protruding-mouth	swim bladder	ISH	Fig. 3
acta2	WT	standard conditions	Pec-fin	swim bladder	ISH	Fig. 3
acta2	WT	standard conditions	Day 4	swim bladder	ISH	Fig. 3
myh10	WT	standard conditions	Long-pec to Day 4	gut	ISH	Fig. 4
myh10	WT	standard conditions	Long-pec to Day 4	swim bladder	ISH	Fig. 4

1 - 10 of 18

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Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Georgijevic et al., 2007 ZDB-PUB-070513-6 PMID:17474123

Spatiotemporal expression of smooth muscle markers in developing zebrafish gut

Georgijevic et al., 2007

ZDB-PUB-070513-6
PMID:17474123