PUBLICATION

Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation

Authors
Huang, H., Lu, F.I., Jia, S., Meng, S., Cao, Y., Wang, Y., Ma, W., Yin, K., Wen, Z., Peng, J., Thisse, C., Thisse, B., and Meng, A.
ID
ZDB-PUB-070303-6
Date
2007
Source
Development (Cambridge, England)   134(5): 979-988 (Journal)
Registered Authors
Cao, Ying, Huang, Huizhe, Jia, Shunji, Lu, Fu-I, Meng, Anming, Peng, Jinrong, Thisse, Bernard, Thisse, Christine, Wen, Zilong
Keywords
Zebrafish, Epiboly, Convergent extension, c-Src, Angiomotin-like2
MeSH Terms
  • Actin Cytoskeleton/physiology
  • Actins/physiology
  • Amino Acid Sequence
  • Animals
  • Body Patterning
  • Cell Movement/physiology
  • Embryo, Nonmammalian/physiology
  • Endosomes/physiology
  • Fibroblast Growth Factors/physiology
  • Membrane Proteins/genetics
  • Membrane Proteins/physiology*
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Transport
  • Pseudopodia/physiology
  • Signal Transduction
  • Vesicular Transport Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • rhoB GTP-Binding Protein/metabolism
  • src-Family Kinases/metabolism*
PubMed
17293535 Full text @ Development
Abstract
Angiomotin (Amot), the founding member of the Motin family, is involved in angiogenesis by regulating endothelial cell motility, and is required for visceral endoderm movement in mice. However, little is known about biological functions of the other two members of the Motin family, Angiomotin-like1 (Amotl1) and Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish amotl2 is expressed maternally and in restricted cell types zygotically. Knockdown of amotl2 expression delays epiboly and impairs convergence and extension movement, and amotl2-deficient cells in mosaic embryos fail to migrate properly. This coincides with loss of membrane protrusions and disorder of F-actin. Amotl2 partially co-localizes with RhoB-or EEA1-positive endosomes and the non-receptor tyrosine kinase c-Src. We further demonstrate that Amotl2 interacts preferentially with and facilitates outward translocation of the phosphorylated c-Src, which may in turn regulate the membrane architecture. These data provide the first evidence that amotl2 is essential for cell movements in vertebrate embryos.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes