ZFIN ID: ZDB-PUB-061108-19
Differential Regulation of Germline mRNAs in Soma and Germ Cells by Zebrafish miR-430
Mishima, Y., Giraldez, A.J., Takeda, Y., Fujiwara, T., Sakamoto, H., Schier, A.F., and Inoue, K.
Date: 2006
Source: Current biology : CB   16(21): 2135-2142 (Journal)
Registered Authors: Giraldez, Antonio, Inoue, Kunio, Mishima, Yuichiro, Schier, Alexander
Keywords: none
MeSH Terms:
  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Gene Expression Regulation, Developmental*
  • Germ Cells/metabolism*
  • MicroRNAs/genetics*
  • Molecular Sequence Data
  • Polyadenylation
  • RNA Interference
  • RNA, Messenger/metabolism*
  • RNA-Binding Proteins
  • Ribonuclease III/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 17084698 Full text @ Curr. Biol.
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ABSTRACT
Early in development, primordial germ cells (PGCs) are set aside from somatic cells and acquire a unique gene-expression program . The mechanisms underlying germline-specific gene expression are largely unknown. Nanos expression is required during germline development and is posttranscriptionally restricted to PGCs . Here we report that the microRNA miR-430 targets the 3' untranslated region (UTR) of nanos1 during zebrafish embryogenesis. A miR-430 target site within the nanos1 3' UTR reduces poly(A) tail length, mRNA stability, and translation. Repression is disrupted in maternal-zygotic dicer mutants (MZdicer), which lack mature miRNAs , and is restored by injection of processed miR-430. Although miR-430 represses other genes equally in germline and soma, specific regions in the nanos1 3' UTR compensate for microRNA-mediated repression in PGCs and allow germline-specific expression. We show that the 3' UTR of an additional PGC-specific gene, TDRD7, is also targeted by miR-430. These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression.
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