PUBLICATION

Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya

Authors
Oishi, I., Kawakami, Y., Raya, A., Callol-Massot, C., and Izpisúa Belmonte, J.C.
ID
ZDB-PUB-061020-8
Date
2006
Source
Nature Genetics   38(11): 1316-1322 (Journal)
Registered Authors
Izpisúa Belmonte, Juan Carlos, Kawakami, Yasuhiko, Oishi, Isao, Raya, Angel
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/genetics*
  • Body Patterning/physiology
  • Cilia/genetics*
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Intracellular Signaling Peptides and Proteins/genetics*
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Organogenesis/genetics
  • Signal Transduction
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
PubMed
17013396 Full text @ Nat. Genet.
Abstract
Primary cilia are microtubule-based organelles that project from the surface of nearly every animal cell. Although important functions of primary cilia in morphogenesis and tissue homeostasis have been identified, the mechanisms that control the formation of primary cilia are not understood. Here we characterize a zebrafish gene, termed duboraya (dub), that is essential for ciliogenesis. Knockdown of dub in zebrafish embryos results in both defects in primary cilia formation in Kupffer's vesicle and randomization of left-right organ asymmetries. We show that, at the molecular level, the function of dub in ciliogenesis is regulated by phosphorylation, which in turn depends on Frizzled-2-mediated noncanonical Wnt signaling. We also provide evidence that, at the cellular level, dub function is essential for actin organization in the cells lining Kupffer's vesicle. Taken together, our findings identify a molecular factor that links noncanonical Wnt signaling with the control of left-right axis specification, and provide an entry point for analyzing the mechanisms that regulate primary cilia formation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes