PUBLICATION
Cloning of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of HSF2 and HSF1 mRNA expression in brain tissues
- Authors
- Yeh, F.L., Hsu, L.Y., Lin, B.A., Chen, C.F., Li, I.C., Tsai, S.H., and Hsu, T.
- ID
- ZDB-PUB-060906-10
- Date
- 2006
- Source
- Biochimie 88(12): 1983-1988 (Journal)
- Registered Authors
- Hsu, Todd
- Keywords
- Heat shock protein, Heat shock element, Heat shock factor, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Brain/embryology
- Brain/metabolism*
- Electrophoretic Mobility Shift Assay
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Heat-Shock Proteins/genetics
- Heat-Shock Proteins/metabolism
- In Situ Hybridization
- Male
- Molecular Sequence Data
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 16938384 Full text @ Biochimie
Citation
Yeh, F.L., Hsu, L.Y., Lin, B.A., Chen, C.F., Li, I.C., Tsai, S.H., and Hsu, T. (2006) Cloning of zebrafish (Danio rerio) heat shock factor 2 (HSF2) and similar patterns of HSF2 and HSF1 mRNA expression in brain tissues. Biochimie. 88(12):1983-1988.
Abstract
The transcriptional activation of heat shock protein (HSP) genes is initiated by the binding of heat shock factors (HSFs) to heat shock elements (HSEs) located at the promotor regions. Multiple HSFs exist in larger eukaryotic organisms in order to sense various types of stress signals. Here we report the cloning of zebrafish (Danio rerio) HSF2 (zHSF2) cDNA (GenBank accession number ) that has an open reading frame of 1470 nucleotides, encoding a polypeptide of 489 amino acids. Domain architecture analysis of the deduced zHSF2 sequence revealed the presence of a DNA-binding domain at the N-terminal end, an adjacent oligomerization domain and a vertebrate heat shock transcription factor domain. Amino acid alignment showed a 70% sequence identity between zHSF2 and human or mouse HSF2, while only a 45% identity was found between zHSF1a and zHSF2. Recombinant zHSF2 bound with a very high specificity to HSEs arranged as inverted arrays of 5'-nGAAn-3', as replacing one GAA with GTA almost abolished the formation of HSE-binding complex. Similar patterns of zHSF1a and zHSF2 mRNA expression in the brain regions of developing zebrafish were detected by whole mount in situ hybridization and paraffin sectioning, suggesting that most of the two HSF gene activities were controlled by a common mechanism during the embryonic development of zebrafish. The levels of both zHSF1a and zHSF2 mRNA in zebrafish tissues were moderately increased after heat stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping