ZFIN ID: ZDB-PUB-060816-7
Zebrafish promoter microarrays identify actively transcribed embryonic genes
Wardle, F.C., Odom, D.T., Bell, G.W., Yuan, B., Danford, T.W., Wiellette, E.L., Herbolsheimer, E., Sive, H.L., Young, R.A., and Smith, J.C.
Date: 2006
Source: Genome biology   7(8): R71 (Journal)
Registered Authors: Sive, Hazel, Smith, Jim, Wardle, Fiona, Wiellette, Elizabeth
Keywords: none
Microarrays: GEO:GSE4863
MeSH Terms:
  • Animals
  • Chromatin Immunoprecipitation
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes/genetics*
  • Genomics/methods
  • Histones/genetics
  • Oligonucleotide Array Sequence Analysis
  • Promoter Regions, Genetic/genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 16889661 Full text @ Genome Biol.
ABSTRACT: We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
Lab website for supplementary data.