PUBLICATION
Neurotoxicity assessment using zebrafish
- Authors
- Parng, C., Roy, N.M., Ton, C., Lin, Y., and McGrath, P.
- ID
- ZDB-PUB-060616-50
- Date
- 2007
- Source
- Journal of Pharmacological and Toxicological Methods 55(1): 103-112 (Journal)
- Registered Authors
- McGrath, Patricia, Parng, Chuenlei, Roy, Nicole, Ton, Christopher
- Keywords
- Apoptosis, Demyelination, Dopaminergic neurons, Methods, Myelin, Motor neurons, Proliferation, Optic nerves, Oxidation, Quantification, Zebrafish
- MeSH Terms
-
- Acrylamide/toxicity
- Animals
- Anthracenes/toxicity
- Apoptosis/drug effects
- Cell Proliferation/drug effects
- Embryo, Nonmammalian/drug effects
- Ethanol/toxicity
- Glutarates/toxicity
- In Situ Hybridization
- In Situ Nick-End Labeling
- Motor Neurons/drug effects
- Myelin Sheath/drug effects
- Neomycin/toxicity
- Neurons/cytology
- Neurons/drug effects
- Neurotoxins/toxicity*
- Optic Nerve/drug effects
- Oxidopamine/toxicity
- Paclitaxel/toxicity
- Toxicity Tests/methods*
- Tretinoin/toxicity
- Zebrafish
- PubMed
- 16769228 Full text @ J. Pharmacol. Toxicol. Methods
Citation
Parng, C., Roy, N.M., Ton, C., Lin, Y., and McGrath, P. (2007) Neurotoxicity assessment using zebrafish. Journal of Pharmacological and Toxicological Methods. 55(1):103-112.
Abstract
Introduction: Transparency is a unique attribute of zebrafish that permits direct assessment of drug effects on the nervous system using whole mount antibody immunostaining and histochemistry. Methods: To assess pharmacological effects of drugs on the optic nerves, motor neurons, and dopaminergic neurons, we performed whole mount immunostaining and visualized different neuronal cell types in vivo. In addition, we assessed neuronal apoptosis, proliferation, oxidation and the integrity of the myelin sheath using TUNEL staining, immunostaining and in situ hybridization. The number of dopaminergic neurons was examined and morphometric analysis was performed to quantify the staining signals for myelin basic protein and apoptosis. Results: We showed that compounds that induce neurotoxicity in humans caused similar neurotoxicity in zebrafish. For example, ethanol induced defects in optic nerves and motor neurons and affected neuronal proliferation; 6-hydroxydopamine caused neuronal oxidation and dopaminergic neuron loss; acrylamide induced demyelination; taxol, neomycin, TCDD and retinoic acid induced neuronal apoptosis. Discussion: Effects of drug treatment on different neurons can easily be visually assessed and quantified in intact animals. These results support the use of zebrafish as a predictive model for assessing neurotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping