PUBLICATION
Metallothionein gene expression in zebrafish embryo-larvae and ZFL cell-line exposed to heavy metal ions
- Authors
- Chan, K.M., Ku, L.L., Chan, P.C., and Cheuk, W.K.
- ID
- ZDB-PUB-060517-24
- Date
- 2006
- Source
- Marine Environmental Research 62 Suppl 1: S83-87 (Journal)
- Registered Authors
- Chan, King-Ming
- Keywords
- Biomarker, mRNA, Sub-lethal effects, Toxicity
- MeSH Terms
-
- Animals
- Cell Line
- DNA Primers/chemistry
- Embryo, Nonmammalian/drug effects*
- Gene Expression Regulation, Developmental/drug effects*
- Hydrogen Peroxide/toxicity
- Larva/drug effects
- Lethal Dose 50
- Metallothionein/biosynthesis*
- Metallothionein/genetics
- Metals, Heavy/toxicity*
- RNA, Messenger/analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology*
- Zebrafish/growth & development
- PubMed
- 16690118 Full text @ Mar. Environ. Res.
- CTD
- 16690118
Citation
Chan, K.M., Ku, L.L., Chan, P.C., and Cheuk, W.K. (2006) Metallothionein gene expression in zebrafish embryo-larvae and ZFL cell-line exposed to heavy metal ions. Marine Environmental Research. 62 Suppl 1:S83-87.
Abstract
The aim of this study is to investigate the induction of zebrafish metallothionein (zMT) gene expression following the administration of different metal ions using in vivo and in vitro models. The zebrafish embryo-larvae were used for the in vivo study, and MT gene expression was studied during the development from fertilization (8hpf) to embryo-larval stage using real-time PCR. The LC50 values and zMT mRNA levels were also measured in embryo-larvae exposed to various metal ions. The general trend of 24h LC50 values as determined is Cu(2+)
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping